Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli | Ralstonia solanacearum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0222 | - |
UDP-glucuronic acid | pH 7.6, 37°C, recombinant enzyme | Ralstonia solanacearum | |
0.1132 | - |
NAD+ | pH 7.6, 37°C, recombinant enzyme | Ralstonia solanacearum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | 120000 | gel filtration, recombinant enzyme | Ralstonia solanacearum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ralstonia solanacearum | one activity is to decarboxylate UDP-glucuronic acid to UDP-beta-L-threo-pentopyranosyl-4-ulose in the presence of NAD+. The second activity converts UDP-beta-L-threo-pentopyranosyl-4-ulose and NADH to UDP-xylose and NAD+, albeit at a lower rate. Following decarboxylation, there is stereospecific protonation at the C5pro-R position | ? | - |
? | |
UDP-alpha-D-glucuronate + NAD+ | Ralstonia solanacearum | - |
UDP-beta-L-threo-pentapyranos-4-ulose + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ralstonia solanacearum | - |
gene RsU4kpxs | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nicke affinity chromatography and gel filtration | Ralstonia solanacearum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | one activity is to decarboxylate UDP-glucuronic acid to UDP-beta-L-threo-pentopyranosyl-4-ulose in the presence of NAD+. The second activity converts UDP-beta-L-threo-pentopyranosyl-4-ulose and NADH to UDP-xylose and NAD+, albeit at a lower rate. Following decarboxylation, there is stereospecific protonation at the C5pro-R position | Ralstonia solanacearum | ? | - |
? | |
UDP-alpha-D-glucuronate + NAD+ | - |
Ralstonia solanacearum | UDP-beta-L-threo-pentapyranos-4-ulose + CO2 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RsU4kpxs | - |
Ralstonia solanacearum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Ralstonia solanacearum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
60 | activity range, profile overview | Ralstonia solanacearum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.285 | - |
UDP-glucuronic acid | pH 7.6, 37°C, recombinant enzyme | Ralstonia solanacearum | |
0.32 | - |
NAD+ | pH 7.6, 37°C, recombinant enzyme | Ralstonia solanacearum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Ralstonia solanacearum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 11 | activity range, profile overview | Ralstonia solanacearum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Ralstonia solanacearum |
General Information | Comment | Organism |
---|---|---|
evolution | the ancestral enzyme of UDP-xylose synthase and UDP-apiose/UDP-xylose synthase is diverged to two distinct enzymatic activities in early bacteria. This separation gave rise to the current UDP-xylose synthase in animal, fungus, and plant as well as to the plant Uaxs and bacterial ArnA and U4kpxs homologues | Ralstonia solanacearum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
NAD+ | pH 7.6, 37°C, recombinant enzyme | Ralstonia solanacearum | |
12.8 | - |
UDP-glucuronic acid | pH 7.6, 37°C, recombinant enzyme | Ralstonia solanacearum |