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Literature summary for 1.1.1.300 extracted from

  • Marchette, L.D.; Thompson, D.A.; Kravtsova, M.; Ngansop, T.N.; Mandal, M.N.; Kasus-Jacobi, A.
    Retinol dehydrogenase 12 detoxifies 4-hydroxynonenal in photoreceptor cells (2010), Free Radic. Biol. Med., 48, 16-25.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
stable transfection of HEK-293 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
T49M inactive. Mutation is associated with Lebr congenital amaurosis. Mutant is not able to detoxify 4-hydroxynonenal in cells Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
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Mus musculus
-
-

Organism

Organism UniProt Comment Textmining
Mus musculus Q8BYK4 Rdh12
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Mus musculus Q9QYF1 Rdh11
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Source Tissue

Source Tissue Comment Organism Textmining
retina
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Mus musculus
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retina expression in inner segments, cell bodies and synaptic termini of photoreceptors Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-4-hydroxy-2-nonenal + NADPH + H+
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Mus musculus (E)-4-hydroxy-2-nonenol + NADP+
-
?
(E)-4-hydroxy-2-nonenal + NADPH + H+ Rdh12 is able to efficiently detoxify 4-hydroxynonenal in cells, most probably through its ability to reduce it to a nontoxic alcohol Mus musculus (E)-4-hydroxy-2-nonenol + NADP+
-
?

General Information

General Information Comment Organism
physiological function Rdh11 is able to efficiently detoxify 4-hydroxynonenal in cells. Rdh11 protects against 4-hydroxynonenal modification of proteins and 4-hydroxynonenal-induced apoptosis in HEK-293 cells Mus musculus
physiological function Rdh12 is able to efficiently detoxify 4-hydroxynonenal in cells, most probably through its ability to reduce it to a nontoxic alcohol. Cells expressing Rdh12 show significantly less formation of Michael adducts with lysine, histidine, or cysteine residues of proteins thereby inhibiting their physiological functions. Microsomes from retinas of Rdh12 knockout mice form significantly more Michael adducts with microsomal proteins in the presence of 4-hydroxynonenal than wild-type. RDH12 also protects against light-induced apoptosis of photoreceptors Mus musculus