Cloned (Comment) | Organism |
---|---|
expression in CHO-K1 cells | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | recombinant RRD reduces free retinal (not bound with cellular retinol binding protein) with a K0.5 value of 0.0023 mM and a Hill constant of 1.7, and reduces CRBP(I)-bound retinal (2fold molar excess of cellular retinol binding protein(I) at each retinal concentration) with a K0.5 value of 0.0086 mM and a Hill constant of 2.1 | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Mus musculus | 5777 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
male C57BL6J mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | low mRNA expression | Mus musculus | - |
embryo | RRD mRNA expression is initiated on embryo day 7 | Mus musculus | - |
heart | high mRNA expression | Mus musculus | - |
kidney | high mRNA expression | Mus musculus | - |
liver | high mRNA expression | Mus musculus | - |
lung | low mRNA expression | Mus musculus | - |
spleen | low mRNA expression | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
13-cis-retinal + NADPH + H+ | 4fold lower activity than with all-trans-retinal | Mus musculus | 13-cis-retinol + NADP+ | - |
? | |
9-cis-retinal + NADPH + H+ | 60fold lower activity than with all-trans-retinal | Mus musculus | 9-cis-retinol + NADP+ | - |
? | |
all-trans-retinal + NADPH + H+ | - |
Mus musculus | all-trans-retinol + NADP+ | - |
? | |
additional information | recombinant RRD functions with both unbound and CRBP(I) (cellular retinol-binding protein)-bound retinal | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
short-chain dehydrogenase/reductase RRD | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | NADPH is at least 30fold more effective than NADH | Mus musculus |