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Literature summary for 1.1.1.300 extracted from

  • Lee, S.; Belyaeva, O.V.; Kedishvili, N.Y.
    Effect of lipid peroxidation products on the activity of human retinol dehydrogenase 12 (RDH12) and retinoid metabolism (2008), Biochim. Biophys. Acta, 1782, 421-425.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine transfection with retinol dehydrogenase 12 protects cells against nonanal-induced toxicity but is ineffective against 4-hydroxynonenal. 4-Hydroxynonenal strongly inhibits the activities of lecithin:retinol acyl transferase and aldehyde dehydrogenase, resulting in decreased levels of retinyl esters and retinoic acid and accumulation of unesterified retinol Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK-293 cell Homo sapiens

Protein Variants

Protein Variants Comment Organism
I51N site-directed mutagenesis, significant activity in vitro. Dramatically reduced affinity for NADPH results in loss of function within cells Homo sapiens
L99I site-directed mutagenesis, about 30% of wild-type activity Homo sapiens
additional information transfection with retinol dehydrogenase 12 protects cells against nonanal-induced toxicity but is ineffective against 4-hydroxynonenal Homo sapiens
S175P site-directed mutagenesis, no catalytic activity. Protein is stable and abundantly expressed Homo sapiens
T49M site-directed mutagenesis, significant activity in vitro. Dramatically reduced affinity for NADPH results in loss of function within cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Nonanal inhibits the activity towards retinaldehyde Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00006
-
all-trans-retinal wild-type Homo sapiens
0.00028
-
all-trans-retinal mutant T49M Homo sapiens
0.00041
-
all-trans-retinal mutant I51N Homo sapiens
0.0007
-
NADPH wild-type Homo sapiens
0.0032
-
NADP+ wild-type Homo sapiens
0.033
-
NADPH mutant T49M Homo sapiens
0.1
-
NADP+ mutant T49M Homo sapiens
0.105
-
NADPH mutant I51N Homo sapiens
0.35
-
NADP+ mutant I51N Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
all-trans-retinal + NADPH + H+ Homo sapiens
-
all-trans-retinol + NADP+
-
?
additional information Homo sapiens although bi-directional in vitro, in living cells, RDH12 acts exclusively as a retinaldehyde reductase, shifting the retinoid homeostasis toward the increased levels of retinol and decreased levels of bioactive retinoic acid. The retinaldehyde reductase activity of RDH12 protects the cells from retinaldehyde-induced cell death ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isoform RDH12
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-retinal + NADPH + H+
-
Homo sapiens all-trans-retinol + NADP+
-
?
additional information although bi-directional in vitro, in living cells, RDH12 acts exclusively as a retinaldehyde reductase, shifting the retinoid homeostasis toward the increased levels of retinol and decreased levels of bioactive retinoic acid. The retinaldehyde reductase activity of RDH12 protects the cells from retinaldehyde-induced cell death Homo sapiens ?
-
?
retinal + NADH
-
Homo sapiens retinol + NAD+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
all-trans-retinal wild-type Homo sapiens
0.21
-
all-trans-retinal mutant I51N Homo sapiens
0.4
-
all-trans-retinal mutant T49M Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens
NADPH
-
Homo sapiens