Literature summary for 1.1.1.300 extracted from

Belyaeva, O.V.; Stetsenko, A.V.; Nelson, P.; Kedishvili, N.Y.
Properties of short-chain dehydrogenase/reductase RalR1: characterization of purified enzyme, its orientation in the microsomal membrane, and distribution in human tissues and cell lines (2003), Biochemistry, 42, 14838-14845.

Search for more literature for 1.1.1.300

Cloned(Commentary)

Cloned (Comment)	Organism
production of recombinant His6-tagged enzyme in insect cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
R25G/K26I	The mutation allows the enzyme to flip its orientation in the membrane. The mutant is glycosylated in intact cells.	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00012	-	all-trans-retinal	purified recombinant enzyme	Homo sapiens
0.0002	-	all-trans-retinal	membrane-bound wild-type	Homo sapiens
0.0004	-	NADP+	membrane-bound wild-type	Homo sapiens
0.00047	-	NADPH	purified recombinant enzyme	Homo sapiens
0.00048	-	NADPH	membrane-bound wild-type	Homo sapiens
0.0006	-	all-trans-retinol	purified recombinant enzyme	Homo sapiens
0.0007	-	all-trans-retinol	membrane-bound wild-type	Homo sapiens
0.001	-	NADP+	purified recombinant enzyme	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	model of enzyme insertion into the membrane	Homo sapiens	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35400	-	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
all-trans-retinol + NADP+	Homo sapiens	the enzyme plays a role in retinoid metabolism	all-trans-retinal + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
no glycoprotein	the wild-type enzyme is not glycosylated	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Homo sapiens	-
jejunum	-	Homo sapiens	-
kidney	-	Homo sapiens	-
liver	-	Homo sapiens	-
lung	-	Homo sapiens	-
additional information	not: skeletal muscle and heart	Homo sapiens	-
prostate gland	-	Homo sapiens	-
testis	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.63	-	eluate after purification, the specific activity of the purified enzyme with all-trans-retinal as substrate is 13fold higher than that of the microsomal preparation of wild-type	Homo sapiens

Storage Stability

Storage Stability	Organism
-80°C, buffer containing 1 mM 1,2-diheptanoyl-sn-glycero-3-phosphocholine, stable for several months	Homo sapiens
8°C, in a refrigerator, the enzyme is nearly fully active for at least one month	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
all-trans-retinol + NADP+	-	Homo sapiens	all-trans-retinal + NADPH + H+	-	r
all-trans-retinol + NADP+	the enzyme plays a role in retinoid metabolism	Homo sapiens	all-trans-retinal + NADPH + H+	-	r

Synonyms

Synonyms	Comment	Organism
RalR1	-	Homo sapiens
retinal reductase 1	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.2	-	all-trans-retinol	purified recombinant enzyme	Homo sapiens
0.3	-	all-trans-retinal	purified recombinant enzyme	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Homo sapiens
NADPH	-	Homo sapiens

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Release 2023.1 (January 2023)