Cloned (Comment) | Organism |
---|---|
- |
Alcaligenes faecalis |
Crystallization (Comment) | Organism |
---|---|
in the presence of the substrate D-3-hydroxybutyrate and the cofactor NAD+ at the optimum pH for the catalytic reaction. At 277 and 293 K using the hanging-drop vapour-diffusion method, to 2.3 A resolution. Structure is isomorphous to that of the complex with the substrate analogue acetate | Alcaligenes faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | D0VWQ0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Alcaligenes faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-3-hydroxybutyrate + NAD+ | the reversible reactions occur by shuttle movements of a hydrogen negative ion from the C3 atom of the substrate to the C4 atom of NAD+ and from the C4 atom of NADH to the C3 atom of the product. The reaction may be further coupled to the withdrawal of a proton from the hydroxyl group of the substrate by the ionized Tyr155 residue | Alcaligenes faecalis | acetoacetate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Alcaligenes faecalis |
Synonyms | Comment | Organism |
---|---|---|
HBDH | - |
Alcaligenes faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Alcaligenes faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Alcaligenes faecalis | |
NADH | - |
Alcaligenes faecalis |