Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21 | Corynebacterium pekinense |
Protein Variants | Comment | Organism |
---|---|---|
L200F | site-directed mutagenesis, compared to mutant L200F, the double mutant shows 2 degree higher optimum temperature, 1.24 times higher activity, but the same pH optimum of pH 7.5 as mutant L200F. Both mutants L200F/D215K and L200F show good resistance to organic solvents and metal ions | Corynebacterium pekinense |
L200F/D215A | site-directed mutagenesis | Corynebacterium pekinense |
L200F/D215E | site-directed mutagenesis | Corynebacterium pekinense |
L200F/D215G | site-directed mutagenesis | Corynebacterium pekinense |
L200F/D215K | site-directed mutagenesis, compared to mutant L200F, the double mutant shows 2 dgree higher optimum temperature, 1.24 times higher activity, but the same pH optimum of pH 7.5 as mutant L200F. Both mutants L200F/D215K and L200F show good resistance to organic solvents and metal ions | Corynebacterium pekinense |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | both mutants L200F/D215K and L200F show good resistance to organic solvents and metal ions | Corynebacterium pekinense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium pekinense | - |
- |
- |
Corynebacterium pekinense AS1.299 | - |
- |
- |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
mutant L200F | Corynebacterium pekinense |
37 | - |
mutant L200F/D215K | Corynebacterium pekinense |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life of mutant L200F/D215K is 4.16 h, of mutant L200F 3.71 h | Corynebacterium pekinense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
mutants L200F/D215K and L200F | Corynebacterium pekinense |