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Literature summary for 1.1.1.3 extracted from

  • Navratna, V.; Gopal, B.
    Crystallization and preliminary X-ray diffraction studies of Staphylococcus aureus homoserine dehydrogenase (2013), Acta Crystallogr. Sect. F, 69, 1216-1219.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
gene hom, recombinant expression of His-tagged enzyme in Echerichia coli strain Rosetta (DE3) pLysS Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme, crystallization at different pH values in the range of pH 6.0-8.5, hanging drop and sitting drop methods of vapour diffusion, 8 mg/ml protein solution is mixed with reservoir solution, different conditions involving addition of 0.2 M magnesium acetate and PEG 3350 or 8000, and 3.5% glycerol, overview. X-ray diffraction structure determination and analysis at 2.1-2.2 A resolution Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Staphylococcus aureus 5618
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-homoserine + NADP+ Staphylococcus aureus
-
 L-aspartate 4-semialdehyde + NADPH + H+
-
 r
L-homoserine + NADP+ Staphylococcus aureus COL
-
 L-aspartate 4-semialdehyde + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus A0A0H2WVX4
-
-
Staphylococcus aureus COL A0A0H2WVX4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Echerichia coli strain Rosetta (DE3) pLysS by nickel affinty chromatography and gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homoserine + NADP+
-
 Staphylococcus aureus L-aspartate 4-semialdehyde + NADPH + H+
-
 r
L-homoserine + NADP+
-
 Staphylococcus aureus COL L-aspartate 4-semialdehyde + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
hom
-
 Staphylococcus aureus
HSD
-
 Staphylococcus aureus
SACOL1362
-
 Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Staphylococcus aureus
NADPH
-
 Staphylococcus aureus

General Information

General Information Comment Organism
metabolism homoserine dehydrogenase (HSD) is an oxidoreductase that is involved in the reversible conversion of L-aspartate semialdehyde to L-homoserine in a dinucleotide cofactor-dependent reduction reaction. HSD is thus a crucial intermediate enzyme linked to the biosynthesis of several essential amino acids such as lysine, methionine, isoleucine and threonine Staphylococcus aureus
physiological function the enzyme is involved in cell-wall maintenance and essential amino acid biosynthesis. Homoserine dehydrogenase catalyzes a reaction at the branch point of the pathway leading to lysine biosynthesis. This pathway is also referred to as the diaminopimelate (dap) pathway Staphylococcus aureus