Cloned (Comment) | Organism |
---|---|
gene hom1, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), recombinant overexpression of constructs comprising the enzyme with homoserine kinase, aspartate kinase, acetohydroxy acid synthase, or threonine dehydratase, overview | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
G378S | construction of a homoserine dehydrogenase mutant HDG378S, encoded by hom1, in Corynebacterium glutamicum strain IWJ001, one of the best L-isoleucine producing strains. Strain HDG378S is partially resistant to L-threonine with the half maximal inhibitory concentration between 12 and 14 mM. Overexpression of lysC1, hom1 and thrB1 increased L-threonine and L-lysine production in Corynebacterium glutamicum ATCC13869 by 96folds and 21.2folds, respectively, overview | Corynebacterium glutamicum |
additional information | releasing the enzymes of the L-threonine biosynthesis pathway from feedback control and coordinating their expression plays a pivotal role in engineering Corynebacterium glutamicum into L-isoleucine producers, construction of transgenic Corynebacterium glutamicum with deregulated L-threonine biosynthesis pathway enzymes for enhanced L-isoleucine biosynthesis | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-threonine | the enzyme activity is subjected to feedback regulation by L-threonine | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homoserine + NADP+ | Corynebacterium glutamicum | - |
L-aspartate 4-semialdehyde + NADPH + H+ | - |
r | |
L-homoserine + NADP+ | Corynebacterium glutamicum IWJ001 | - |
L-aspartate 4-semialdehyde + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | P08499 | - |
- |
Corynebacterium glutamicum IWJ001 | P08499 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homoserine + NADP+ | - |
Corynebacterium glutamicum | L-aspartate 4-semialdehyde + NADPH + H+ | - |
r | |
L-homoserine + NADP+ | - |
Corynebacterium glutamicum IWJ001 | L-aspartate 4-semialdehyde + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
hom | - |
Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Corynebacterium glutamicum | |
NADPH | - |
Corynebacterium glutamicum |
General Information | Comment | Organism |
---|---|---|
metabolism | homoserine dehydrogenase is a key enzyme in the L-threonine pathway | Corynebacterium glutamicum |