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Literature summary for 1.1.1.3 extracted from

  • Dong, X.; Zhao, Y.; Zhao, J.; Wang, X.
    Characterization of aspartate kinase and homoserine dehydrogenase from Corynebacterium glutamicum IWJ001 and systematic investigation of L-isoleucine biosynthesis (2016), J. Ind. Microbiol. Biotechnol., 43, 873-885.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene hom1, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), recombinant overexpression of constructs comprising the enzyme with homoserine kinase, aspartate kinase, acetohydroxy acid synthase, or threonine dehydratase, overview Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
G378S construction of a homoserine dehydrogenase mutant HDG378S, encoded by hom1, in Corynebacterium glutamicum strain IWJ001, one of the best L-isoleucine producing strains. Strain HDG378S is partially resistant to L-threonine with the half maximal inhibitory concentration between 12 and 14 mM. Overexpression of lysC1, hom1 and thrB1 increased L-threonine and L-lysine production in Corynebacterium glutamicum ATCC13869 by 96folds and 21.2folds, respectively, overview Corynebacterium glutamicum
additional information releasing the enzymes of the L-threonine biosynthesis pathway from feedback control and coordinating their expression plays a pivotal role in engineering Corynebacterium glutamicum into L-isoleucine producers, construction of transgenic Corynebacterium glutamicum with deregulated L-threonine biosynthesis pathway enzymes for enhanced L-isoleucine biosynthesis Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
L-threonine the enzyme activity is subjected to feedback regulation by L-threonine Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-homoserine + NADP+ Corynebacterium glutamicum
-
L-aspartate 4-semialdehyde + NADPH + H+
-
r
L-homoserine + NADP+ Corynebacterium glutamicum IWJ001
-
L-aspartate 4-semialdehyde + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum P08499
-
-
Corynebacterium glutamicum IWJ001 P08499
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homoserine + NADP+
-
Corynebacterium glutamicum L-aspartate 4-semialdehyde + NADPH + H+
-
r
L-homoserine + NADP+
-
Corynebacterium glutamicum IWJ001 L-aspartate 4-semialdehyde + NADPH + H+
-
r

Synonyms

Synonyms Comment Organism
hom
-
Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Corynebacterium glutamicum
NADPH
-
Corynebacterium glutamicum

General Information

General Information Comment Organism
metabolism homoserine dehydrogenase is a key enzyme in the L-threonine pathway Corynebacterium glutamicum