Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21 | Bacillus cereus |
MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: EDTA and ethylene glykol, up to 0.2 mM | Bacillus cereus | |
Zn | 0.2 mM, 60% inhibition | Bacillus cereus | |
Zn2+ | 60% inhibition at 0.2 mM | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, overview | Bacillus cereus | |
additional information | - |
additional information | steady-state kinetic analysis, overview | Bacillus cereus | |
0.25 | - |
NADP+ | pH 8.5, 37°C | Bacillus cereus | |
0.25 | - |
NADP+ | pH 8.5, 37°C, recombinant enzyme | Bacillus cereus | |
2.4 | - |
NAD+ | pH 8.5, 37°C | Bacillus cereus | |
2.4 | - |
NAD+ | pH 8.5, 37°C, recombinant enzyme | Bacillus cereus | |
16.8 | - |
3-hydroxypropionate | pH 8.5, 37°C | Bacillus cereus | |
16.8 | - |
3-hydroxypropanoate | pH 8.5, 37°C | Bacillus cereus | |
16.8 | - |
3-hydroxypropionate | pH 8.5, 37°C, recombinant enzyme | Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Bacillus cereus | |
additional information | up to 0.2 mM, no effect on activity: MnSO4, CuSO4, CaCl2, MgSO4, and FeSO4 | Bacillus cereus | |
additional information | no metal ion requirement, no effects by 0.2 mM MnSO4, CuSO4, CaCl2, MgSO4, and FeSO4 | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Bacillus cereus |
32000 | - |
x * 32000, recombinant enzyme, SDS-PAGE | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxypropanoate + NADP+ | Bacillus cereus | - |
3-oxopropanoate + NADPH + H+ | - |
r | |
3-hydroxypropanoate + NADP+ | Bacillus cereus | - |
malonate semialdehyde + NADPH + H+ | - |
r | |
additional information | Bacillus cereus | MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity | ? | - |
? | |
additional information | Bacillus cereus | the enzyme is a 3-hydroxyisobutyrate dehydrogenase, 3-HIBADH, EC1.1.1.31, that also utilizes 3-hydroxypropionate as substrate. It catalyzes not only the oxidation of 3-hydroxyisobutyrate but also of L-serine, D-threonine, and other 3-hydroxyacid derivatives | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
gene MmsB | - |
Bacillus cereus | - |
- |
- |
Bacillus cereus | - |
MmsB gene | - |
Bacillus cereus ATCC 14579 | - |
gene MmsB | - |
Bacillus cereus ATCC 14579 | - |
- |
- |
Bacillus cereus ATCC 14579 | - |
MmsB gene | - |
Purification (Comment) | Organism |
---|---|
native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography | Bacillus cereus |
recombinant enzyme from Escherichia coli strain BL21 96fold by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography, followed by ultrafiltration | Bacillus cereus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.7 | - |
pH 8.5, 37°C | Bacillus cereus |
8.8 | - |
purified recombinant enzyme, pH 8.5, 37°C | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxypropanoate + NAD+ | - |
Bacillus cereus | malonate semialdehyde + NADH + H+ | - |
r | |
3-hydroxypropanoate + NAD+ | - |
Bacillus cereus | malonate-semialdehyde + NADH + H+ | - |
? | |
3-hydroxypropanoate + NADP+ | 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, additionally exhibits 3-hydroxypropionate dehydrogenase activity | Bacillus cereus | malonate-semialdehyde + NADPH + H+ | - |
? | |
3-hydroxypropanoate + NADP+ | - |
Bacillus cereus | 3-oxopropanoate + NADPH + H+ | - |
r | |
3-hydroxypropanoate + NADP+ | - |
Bacillus cereus | malonate semialdehyde + NADPH + H+ | - |
r | |
3-hydroxypropanoate + NADP+ | - |
Bacillus cereus | malonate semialdehyde + NADPH + H+ | spectrophotometric product determination | r | |
additional information | MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity | Bacillus cereus | ? | - |
? | |
additional information | the enzyme is a 3-hydroxyisobutyrate dehydrogenase, 3-HIBADH, EC1.1.1.31, that also utilizes 3-hydroxypropionate as substrate. It catalyzes not only the oxidation of 3-hydroxyisobutyrate but also of L-serine, D-threonine, and other 3-hydroxyacid derivatives | Bacillus cereus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Bacillus cereus |
? | x * 32000, recombinant enzyme, SDS-PAGE | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
3-HIBADH | - |
Bacillus cereus |
3-hydroxyisobutyrate dehydrogenase | - |
Bacillus cereus |
3-hydroxypropionate dehydrogenase | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus cereus |
37 | - |
assay at | Bacillus cereus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 45 | 90% of maximal activity within this range | Bacillus cereus |
35 | 45 | activity range | Bacillus cereus |
35 | 45 | more than 90% of maximum activity | Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | purified enzyme, over 90% activity remaining after 30 min | Bacillus cereus |
45 | - |
30 min, stable | Bacillus cereus |
45 | - |
30 min, purified enzyme, completely stable | Bacillus cereus |
55 | - |
3 min, about 40% residual activity | Bacillus cereus |
55 | - |
3 min, purified enzyme, significant denaturation and inactivation | Bacillus cereus |
55 | - |
purified enzyme, 30 min, partial denaturation | Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
3-hydroxypropionate | pH 8.5, 37°C | Bacillus cereus | |
0.21 | - |
3-hydroxypropanoate | pH 8.5, 37°C | Bacillus cereus | |
0.21 | - |
3-hydroxypropionate | pH 8.5, 37°C, recombinant enzyme | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Bacillus cereus |
8.8 | 9 | - |
Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | activity range | Bacillus cereus |
8.2 | - |
about 30% of maximum acticity | Bacillus cereus |
9 | - |
about 65% of maximum activity | Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NADP+ is preferred over NAD+ | Bacillus cereus | |
NAD+ | more active with NADP+ than NAD+ | Bacillus cereus | |
NADH | - |
Bacillus cereus | |
NADP+ | - |
Bacillus cereus | |
NADP+ | NADP+ is preferred over NAD+ | Bacillus cereus | |
NADP+ | more active with NADP+ than NAD+ | Bacillus cereus | |
NADPH | - |
Bacillus cereus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is a 3-hydroxyisobutyrate dehydrogenase, 3-HIBADH, EC1.1.1.31, that also utilizes 3-hydroxypropionate as substrate. It catalyzes not only the oxidation of 3-hydroxyisobutyrate but also of L-serine, D-threonine, and other 3-hydroxyacid derivatives. 3-HIBADH may have the similar function to 3-hydroxypropionate dehydrogenase in vivo and be the key enzyme in an autotrophic CO2 fixation pathway, the 3-hydroxypropionate cycle | Bacillus cereus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0125 | - |
3-hydroxypropionate | pH 8.5, 37°C | Bacillus cereus | |
0.0125 | - |
3-hydroxypropanoate | pH 8.5, 37°C | Bacillus cereus | |
0.0125 | - |
3-hydroxypropionate | pH 8.5, 37°C, recombinant enzyme | Bacillus cereus |