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Literature summary for 1.1.1.289 extracted from

  • Greenberg, J.R.; Price, N.P.; Oliver, R.P.; Sherman, F.; Rustchenko, E.
    Candida albicans SOU1 encodes a sorbose reductase required for L-sorbose utilization (2005), Yeast, 22, 957-969.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1873
-
D-fructose K0.5-value, positive cooperativity Candida albicans
3953
-
L-sorbose pH 7.5, 25°C Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
4 * 31000, SDS-PAGE and calculated Candida albicans
124000
-
MALDI-TOF Candida albicans

Organism

Organism UniProt Comment Textmining
Candida albicans
-
recombinant enzyme with FLAG-tag
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme with FLAG-tag Candida albicans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.93
-
substrate D-sorbitol, pH 6.2, 25°C Candida albicans
33.1
-
substrate D-fructose, pH 6.2, 25°C Candida albicans
145
-
substrate L-sorbose, pH 6.2, 25°C Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADPH less effective than reaction with L-sorbose Candida albicans D-mannitol + NADP+
-
?
D-sorbitol + NADP+ low efficiency Candida albicans L-sorbose + NADPH + H+
-
?
L-sorbose + NADPH + H+
-
Candida albicans L-sorbitol + NADP+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 31000, SDS-PAGE and calculated Candida albicans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
2 min, more than 90% inactivation Candida albicans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
154
-
D-fructose pH 7.5, 25°C, positive cooperativity Candida albicans
782
-
L-sorbose pH 7.5, 25°C, Michaelis-Menten kinetics Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Candida albicans

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Candida albicans

pI Value

Organism Comment pI Value Maximum pI Value
Candida albicans isoelectric focusing and calculated
-
4.8