KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1873 | - |
D-fructose | K0.5-value, positive cooperativity | Candida albicans | |
3953 | - |
L-sorbose | pH 7.5, 25°C | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
4 * 31000, SDS-PAGE and calculated | Candida albicans |
124000 | - |
MALDI-TOF | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
recombinant enzyme with FLAG-tag | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme with FLAG-tag | Candida albicans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.93 | - |
substrate D-sorbitol, pH 6.2, 25°C | Candida albicans |
33.1 | - |
substrate D-fructose, pH 6.2, 25°C | Candida albicans |
145 | - |
substrate L-sorbose, pH 6.2, 25°C | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADPH | less effective than reaction with L-sorbose | Candida albicans | D-mannitol + NADP+ | - |
? | |
D-sorbitol + NADP+ | low efficiency | Candida albicans | L-sorbose + NADPH + H+ | - |
? | |
L-sorbose + NADPH + H+ | - |
Candida albicans | L-sorbitol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 31000, SDS-PAGE and calculated | Candida albicans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
2 min, more than 90% inactivation | Candida albicans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
154 | - |
D-fructose | pH 7.5, 25°C, positive cooperativity | Candida albicans | |
782 | - |
L-sorbose | pH 7.5, 25°C, Michaelis-Menten kinetics | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Candida albicans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Candida albicans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Candida albicans | isoelectric focusing and calculated | - |
4.8 |