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Literature summary for 1.1.1.284 extracted from

  • Bateman, R.L.; Rauh, D.; Tavshanjian, B.; Shokat, K.M.
    Human carbonyl reductase 1 is an S-nitrosoglutathione reductase (2008), J. Biol. Chem., 283, 35756-35762.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of CBR1 in complex with NADP+ or a substrate mimic (3-(1-tert-butyl-4-amino-1H-indazol-3-yl)phenol) in complex with GSH and the catalytically inert GSH conjugate hydroxymethylglutathione, by vapor diffusion method by growth in the presence of 3-(1-tert-butyl-4-amino-1H-indazol-3-yl)phenol Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3-(1-tert-butyl-4-amino-1H-indazol-3-yl)phenol CBR1 inhibitor, does not inhibit NADH-dependent S-nitrosoglutathione reduction Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0301
-
S-nitrosoglutathione
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-nitrosoglutathione + NAD(P)H + H+
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
carbonyl reductase 1
-
Homo sapiens
CBR1
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.5
-
S-nitrosoglutathione
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADH
-
Homo sapiens
NADPH CBR1 is a NADPH-dependent S-nitrosoglutathione reducing enzyme Homo sapiens

General Information

General Information Comment Organism
physiological function CBR1 may be involved in regulation of tissue levels of S-nitrosoglutathione, but inhibits ca. 30% of the NADPH-dependent activity Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
249.2
-
S-nitrosoglutathione
-
Homo sapiens