Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystal structures of CBR1 in complex with NADP+ or a substrate mimic (3-(1-tert-butyl-4-amino-1H-indazol-3-yl)phenol) in complex with GSH and the catalytically inert GSH conjugate hydroxymethylglutathione, by vapor diffusion method by growth in the presence of 3-(1-tert-butyl-4-amino-1H-indazol-3-yl)phenol | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(1-tert-butyl-4-amino-1H-indazol-3-yl)phenol | CBR1 inhibitor, does not inhibit NADH-dependent S-nitrosoglutathione reduction | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0301 | - |
S-nitrosoglutathione | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-nitrosoglutathione + NAD(P)H + H+ | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase 1 | - |
Homo sapiens |
CBR1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.5 | - |
S-nitrosoglutathione | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Homo sapiens | |
NADPH | CBR1 is a NADPH-dependent S-nitrosoglutathione reducing enzyme | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | CBR1 may be involved in regulation of tissue levels of S-nitrosoglutathione, but inhibits ca. 30% of the NADPH-dependent activity | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
249.2 | - |
S-nitrosoglutathione | - |
Homo sapiens |