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Literature summary for 1.1.1.284 extracted from

  • Staab, C.A.; Alander, J.; Brandt, M.; Lengqvist, J.; Morgenstern, R.; Grafstroem, R.C.; Hoeoeg, J.O.
    Reduction of S-nitrosoglutathione by alcohol dehydrogenase 3 is facilitated by substrate alcohols via direct cofactor recycling and leads to GSH-controlled formation of glutathione transferase inhibitors (2008), Biochem. J., 413, 493-504.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
formaldehyde in the presence of NAD+ and initial absence of NADH, ADH3-mediated S-nitrosoglutathione reduction is triggered by the presence of formaldehyde Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00012
-
S-hydroxymethylglutathione in 0.1 M potassium phosphate (pH 7.5), at 25°C Homo sapiens
0.0111
-
S-nitrosoglutathione in 0.1 M potassium phosphate (pH 7.5), at 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Blue-Sepharose 6 column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + glutathione + NAD+
-
Homo sapiens S-formylglutathione + NADH + H+
-
r
S-hydroxymethylglutathione + NAD+
-
Homo sapiens S-formylglutathione + NADH + H+
-
r
S-nitrosoglutathione + NADH + H+
-
Homo sapiens S-amino-L-glutathione + NAD+ + ?
-
r

Synonyms

Synonyms Comment Organism
ADH3
-
Homo sapiens
alcohol dehydrogenase 3 exhibits dual function as S-nitrosoglutathione reductase and glutathione-dependent formaldehyde dehydrogenase Homo sapiens
GSNO reductase
-
Homo sapiens
GSNOR
-
Homo sapiens
S-nitrosoglutathione reductase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
S-hydroxymethylglutathione in 0.1 M potassium phosphate (pH 7.5), at 25°C Homo sapiens
20
-
S-nitrosoglutathione in 0.1 M potassium phosphate (pH 7.5), at 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens