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Literature summary for 1.1.1.284 extracted from

  • Demkiv, O.M.; Paryzhak, S.Y.; Gayda, G.Z.; Sibirny, V.A.; Gonchar, M.V.
    Formaldehyde dehydrogenase from the recombinant yeast Hansenula polymorpha: isolation and bioanalytic application (2007), FEMS Yeast Res., 7, 1153-1159.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information formaldehyde induces the enzyme expression Ogataea angusta

Application

Application Comment Organism
degradation the enzyme is useful in elimination of formaldehyde, a toxic mutagen mediating apoptosis in cells, from consumers goods and environment Ogataea angusta
diagnostics the enzyme is useful for determination of formaldehyde in consumers goods and environment Ogataea angusta
environmental protection the enzyme is useful in elimination of formaldehyde, a toxic mutagen mediating apoptosis in cells, from consumers goods and environment Ogataea angusta

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Hansenula polymorpha strain Tf 11-6, method optimization for recombinant large scale production, overview Ogataea angusta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, recombinant enzyme, SDS-PAGE Ogataea angusta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formaldehyde + glutathione + NAD+ Ogataea angusta key enzyme in formaldehyde metabolism in microorganisms S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+ Ogataea angusta NCYC 495 key enzyme in formaldehyde metabolism in microorganisms S-formylglutathione + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
gene FLD1
-
Ogataea angusta NCYC 495
-
gene FLD1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 3.8fold from overproducing Hansenula polymorpha strain Tf 11-6 by two steps of anion exchange chromatography and ammonium sulfate fractionation Ogataea angusta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
native strain Tf 11-6 Ogataea angusta
17
-
purified recombinant enzyme, 25°C Ogataea angusta
27
-
purified recombinant enzyme, 37°C Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + glutathione + NAD+ the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione Ogataea angusta S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+ key enzyme in formaldehyde metabolism in microorganisms Ogataea angusta S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+ the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione Ogataea angusta NCYC 495 S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+ key enzyme in formaldehyde metabolism in microorganisms Ogataea angusta NCYC 495 S-formylglutathione + NADH + H+
-
?
additional information enzyme detection in a coupled reaction of formaldehyde oxidation with formazan production from chromogenic agent NTB Ogataea angusta ?
-
?
additional information enzyme detection in a coupled reaction of formaldehyde oxidation with formazan production from chromogenic agent NTB Ogataea angusta NCYC 495 ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, recombinant enzyme, SDS-PAGE Ogataea angusta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
recombinant enzyme Ogataea angusta

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65 60% of maximal activity at 30°C and 65°C Ogataea angusta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
10 min, purified recombinant enzyme, completely stable Ogataea angusta
55
-
10 min, purified recombinant enzyme, 70% remaining activity Ogataea angusta
60
-
10 min, purified recombinant enzyme, 25% remaining activity Ogataea angusta
70
-
5 min, purified recombinant enzyme, complete inactivation Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 recombinant enzyme Ogataea angusta

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8.5 purified recombinant enzyme, stabel Ogataea angusta