Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | formaldehyde induces the enzyme expression | Ogataea angusta |
Application | Comment | Organism |
---|---|---|
degradation | the enzyme is useful in elimination of formaldehyde, a toxic mutagen mediating apoptosis in cells, from consumers goods and environment | Ogataea angusta |
diagnostics | the enzyme is useful for determination of formaldehyde in consumers goods and environment | Ogataea angusta |
environmental protection | the enzyme is useful in elimination of formaldehyde, a toxic mutagen mediating apoptosis in cells, from consumers goods and environment | Ogataea angusta |
Cloned (Comment) | Organism |
---|---|
overexpression in Hansenula polymorpha strain Tf 11-6, method optimization for recombinant large scale production, overview | Ogataea angusta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, recombinant enzyme, SDS-PAGE | Ogataea angusta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + glutathione + NAD+ | Ogataea angusta | key enzyme in formaldehyde metabolism in microorganisms | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | Ogataea angusta NCYC 495 | key enzyme in formaldehyde metabolism in microorganisms | S-formylglutathione + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | - |
gene FLD1 | - |
Ogataea angusta NCYC 495 | - |
gene FLD1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 3.8fold from overproducing Hansenula polymorpha strain Tf 11-6 by two steps of anion exchange chromatography and ammonium sulfate fractionation | Ogataea angusta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
native strain Tf 11-6 | Ogataea angusta |
17 | - |
purified recombinant enzyme, 25°C | Ogataea angusta |
27 | - |
purified recombinant enzyme, 37°C | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + glutathione + NAD+ | the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione | Ogataea angusta | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | key enzyme in formaldehyde metabolism in microorganisms | Ogataea angusta | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione | Ogataea angusta NCYC 495 | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | key enzyme in formaldehyde metabolism in microorganisms | Ogataea angusta NCYC 495 | S-formylglutathione + NADH + H+ | - |
? | |
additional information | enzyme detection in a coupled reaction of formaldehyde oxidation with formazan production from chromogenic agent NTB | Ogataea angusta | ? | - |
? | |
additional information | enzyme detection in a coupled reaction of formaldehyde oxidation with formazan production from chromogenic agent NTB | Ogataea angusta NCYC 495 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, recombinant enzyme, SDS-PAGE | Ogataea angusta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Ogataea angusta |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | 60% of maximal activity at 30°C and 65°C | Ogataea angusta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
10 min, purified recombinant enzyme, completely stable | Ogataea angusta |
55 | - |
10 min, purified recombinant enzyme, 70% remaining activity | Ogataea angusta |
60 | - |
10 min, purified recombinant enzyme, 25% remaining activity | Ogataea angusta |
70 | - |
5 min, purified recombinant enzyme, complete inactivation | Ogataea angusta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | recombinant enzyme | Ogataea angusta |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | purified recombinant enzyme, stabel | Ogataea angusta |