Cloned (Comment) | Organism |
---|---|
gene Lbuc_0522, recombinant overexpression in Escherichia coli strain BL21(DE3)pLysS | Lentilactobacillus buchneri |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the recombinant enzyme overexpressed in Escherichia coli strain BL21(DE3)pLysS is tolerant against 4% v/v ethanol in the growth medium | Lentilactobacillus buchneri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactaldehyde + NADP+ | Lentilactobacillus buchneri | - |
methylglyoxal + NADPH + H+ | - |
? | |
(S)-lactaldehyde + NADP+ | Lentilactobacillus buchneri NRRL B-30929 | - |
methylglyoxal + NADPH + H+ | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | the recombinant enzyme overexpressed in Escherichia coli strain BL21(DE3)pLysS is tolerant against 4% v/v ethanol in the growth medium | Lentilactobacillus buchneri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentilactobacillus buchneri | - |
- |
- |
Lentilactobacillus buchneri NRRL B-30929 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactaldehyde + NADP+ | - |
Lentilactobacillus buchneri | methylglyoxal + NADPH + H+ | - |
? | |
(S)-lactaldehyde + NADP+ | - |
Lentilactobacillus buchneri NRRL B-30929 | methylglyoxal + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Lbuc_0522 | - |
Lentilactobacillus buchneri |
MG reductase | - |
Lentilactobacillus buchneri |
NADPH-dependent methylglyoxal reductase | - |
Lentilactobacillus buchneri |
General Information | Comment | Organism |
---|---|---|
evolution | MG reductases are highly conserved | Lentilactobacillus buchneri |
physiological function | the enzyme is involved in detoxification of methylglyoxal (MG), a cytotoxic by-product of glycolysis that is identified as a potential glycolytic inhibitor, which inhibits the growth of glucose-fermenting yeast cells by promoting degradation of the glucose sensors. The MG reductase gene has been reported to play a critical role in maintaining redox balance during ethanol fermentation in yeasts | Lentilactobacillus buchneri |