Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates | Saccharomyces cerevisiae | |
dithiothreitol | activates | Saccharomyces cerevisiae | |
glutathione | activates | Saccharomyces cerevisiae | |
L-cysteine | activates | Saccharomyces cerevisiae | |
Triton X-100 | activates | Saccharomyces cerevisiae | |
Tween 80 | activates | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Saccharomyces cerevisiae | |
iodoacetate | - |
Saccharomyces cerevisiae | |
N-dodecanoylsarcosine | - |
Saccharomyces cerevisiae | |
N-ethylmaleimide | - |
Saccharomyces cerevisiae | |
NADP+ | - |
Saccharomyces cerevisiae | |
p-chloromercuribenzoate | - |
Saccharomyces cerevisiae | |
sodium cholate | - |
Saccharomyces cerevisiae | |
sodium dodecylsulfate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
NADPH | - |
Saccharomyces cerevisiae | |
1.54 | - |
Phenylglyoxal | - |
Saccharomyces cerevisiae | |
5.88 | - |
methylglyoxal | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + NADPH | Saccharomyces cerevisiae | similar enzyme with NADPH requirement, no reaction with NAD+, NADH and NADP+ | lactaldehyde + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 6.6% carbohydrate, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ | Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.38 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxal + NADPH | - |
Saccharomyces cerevisiae | glycolaldehyde + NADP+ | - |
ir | |
methylglyoxal + NADPH | similar enzyme with NADPH requirement, no reaction with NAD+, NADH and NADP+ | Saccharomyces cerevisiae | lactaldehyde + NADP+ | - |
ir | |
methylglyoxal + NADPH + H+ | - |
Saccharomyces cerevisiae | L-lactaldehyde + NADP+ | - |
r | |
additional information | NADPH required | Saccharomyces cerevisiae | ? | - |
? | |
phenylglyoxal + NADPH | - |
Saccharomyces cerevisiae | hydroxyphenylacetaldehyde + NADP+ | - |
? |