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Literature summary for 1.1.1.28 extracted from
- Production of D-lactic acid by Corynebacterium glutamicum under oxygen deprivation (2008), Appl. Microbiol. Biotechnol., 78, 449-454.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ldhA, expression in an enzyme-deficient ldhA-null Corynebacterium glutamicum strain resulting in increased production of D-lactate | Escherichia coli |
| gene ldhA, expression in an enzyme-deficient ldhA-null Corynebacterium glutamicum strain resulting in increased production of D-lactate | Lactobacillus delbrueckii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli |  |
| Mg2+ | - |
Lactobacillus delbrueckii | |
| Mn2+ | - |
Escherichia coli | |
| Mn2+ | - |
Lactobacillus delbrueckii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + NADH + H+ | Escherichia coli | - |
(R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | Lactobacillus delbrueckii | - |
(R)-lactate + NAD+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene ldhA | - |
| Lactobacillus delbrueckii | - |
gene ldhA | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of the recombinant enzyme in Corynebacerium glutamicum compared to the wild-type enzyme in Escherichia coli, overview | Escherichia coli |
| additional information | - |
activity of the recombinant enzyme in Corynebacerium glutamicum compared to the wild-type enzyme in Lactobacillus delbrueckii, overview | Lactobacillus delbrueckii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + NADH + H+ | - |
Escherichia coli | (R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | - |
Lactobacillus delbrueckii | (R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | a highly stereospecific reaction leading to 98-99%optical purity of the product | Escherichia coli | (R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | a highly stereospecific reaction leading to 98-99%optical purity of the product | Lactobacillus delbrueckii | (R)-lactate + NAD+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-lactate dehydrogenase | - |
Escherichia coli |
| D-lactate dehydrogenase | - |
Lactobacillus delbrueckii |
| D-LDH | - |
Escherichia coli |
| D-LDH | - |
Lactobacillus delbrueckii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
| 7.5 | - |
assay at | Lactobacillus delbrueckii |
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Release 2023.1 (January 2023)
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