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Literature summary for 1.1.1.28 extracted from

  • Isobe, K.; Koide, Y.; Yokoe, M.; Wakao, N.
    Crystallization and some properties of D-lactate dehydrogenase from Staphylococcus sp. LDH-1 (2002), J. Biosci. Bioeng., 94, 330-335.
    View publication on PubMed
Search for more literature for 1.1.1.28

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: LiCl, MnCl2, NiCl2, CoCl2, MgCl2, CuSO4, AlCl3, hydroxylamaine, hydrazine Staphylococcus sp.
phenylhydrazine up to 45% inhibition Staphylococcus sp.
ZnSO4 1 mM, 80% inhibition, EDTA restores Staphylococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 pyruvate pH 8.0, 25°C Staphylococcus sp.
2.3
-
 2-oxobutyrate pH 8.0, 25°C Staphylococcus sp.
8.5
-
 (R)-lactate pH 8.0, 25°C Staphylococcus sp.
19
-
 DL-2-hydroxybutyrate pH 8.0, 25°C Staphylococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, SDS-PAGE Staphylococcus sp.
64000
-
 gel filtration Staphylococcus sp.

Organism

Organism UniProt Comment Textmining
Staphylococcus sp.
-
 isolated as high producer of enzyme, growth under anaerobic conditions
-
Staphylococcus sp. LDH-1
-
 isolated as high producer of enzyme, growth under anaerobic conditions
-

Purification (Commentary)

Purification (Comment) Organism
-
 Staphylococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1710
-
 pH 8.0, 25°C Staphylococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + NAD+
-
 Staphylococcus sp. pyruvate + NADH
-
 r
(R)-lactate + NAD+
-
 Staphylococcus sp. LDH-1 pyruvate + NADH
-
 r
2-oxobutyrate + NADH 11% of activity with pyruvate Staphylococcus sp. 2-hydroxybutyrate + NAD+
-
 r
2-oxobutyrate + NADH 11% of activity with pyruvate Staphylococcus sp. LDH-1 2-hydroxybutyrate + NAD+
-
 r
DL-2-hydroxybutyrate + NAD+ 3% of activity with (R)-lactate Staphylococcus sp. 2-oxobutyrate + NADH
-
 r
DL-2-hydroxybutyrate + NAD+ 3% of activity with (R)-lactate Staphylococcus sp. LDH-1 2-oxobutyrate + NADH
-
 r
methylpyruvate + NADH 84% of activity with pyruvate Staphylococcus sp. ? + NAD+
-
 r
methylpyruvate + NADH 84% of activity with pyruvate Staphylococcus sp. LDH-1 ? + NAD+
-
 r
additional information specific for NAD+/NADH Staphylococcus sp. ?
-
 ?
additional information specific for NAD+/NADH Staphylococcus sp. LDH-1 ?
-
 ?
pyruvate + NADH + H+
-
 Staphylococcus sp. (R)-lactate + NAD+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Staphylococcus sp.
More N-terminal amino acid sequence Staphylococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Staphylococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 33% of activity at 40°C Staphylococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 substrate pyruvate Staphylococcus sp.
8.2
-
 substrate D-lactate Staphylococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5
-
 Staphylococcus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Staphylococcus sp.
NADH
-
 Staphylococcus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Staphylococcus sp. isoelectric focusing
-
 4.2