Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | strongly reducing enviroment required | Enterococcus faecium | |
acetate | - |
Enterococcus faecium | |
phosphate | - |
Enterococcus faecium |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as thioredoxin fusion protein | Enterococcus faecium |
Crystallization (Comment) | Organism |
---|---|
hexagonal and tetragonal crystal forms, tetragonal form diffracted to 3.0 A resolution | Enterococcus faecium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
NADPH | - |
Enterococcus faecium | |
0.019 | - |
NADH | - |
Enterococcus faecium | |
1.5 | - |
pyruvate | - |
Enterococcus faecium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | Enterococcus faecium | - |
pyruvate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | - |
Enterococcus faecium | pyruvate + NADH | - |
? | |
pyruvate + NADPH + H+ | - |
Enterococcus faecium | (R)-lactate + NADP+ | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Enterococcus faecium | |
NADH | - |
Enterococcus faecium |