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Literature summary for 1.1.1.28 extracted from

  • Stoll, V.S.; Manohar, A.V.; Gillon, W.; MacFarlane, E.L.; Hynes, R.C.; Pai, E.F.
    A thioredoxin fusion protein of VanH, a D-lactate dehydrogenase from Enterococcus faecium: cloning, expression, purification, kinetic analysis, and crystallization (1998), Protein Sci., 7, 1147-1155.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol strongly reducing enviroment required Enterococcus faecium
acetate
-
Enterococcus faecium
phosphate
-
Enterococcus faecium

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as thioredoxin fusion protein Enterococcus faecium

Crystallization (Commentary)

Crystallization (Comment) Organism
hexagonal and tetragonal crystal forms, tetragonal form diffracted to 3.0 A resolution Enterococcus faecium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
NADPH
-
Enterococcus faecium
0.019
-
NADH
-
Enterococcus faecium
1.5
-
pyruvate
-
Enterococcus faecium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-lactate + NAD+ Enterococcus faecium
-
pyruvate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Enterococcus faecium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + NAD+
-
Enterococcus faecium pyruvate + NADH
-
?
pyruvate + NADPH + H+
-
Enterococcus faecium (R)-lactate + NADP+
-
r

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Enterococcus faecium
NADH
-
Enterococcus faecium