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Literature summary for 1.1.1.274 extracted from

  • Kaswurm, V.; Nguyen, T.T.; Maischberger, T.; Kulbe, K.D.; Michlmayr, H.
    Evaluation of the food grade expression systems NICE and pSIP for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum (2013), AMB Express, 3, 7-7.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene dkr, recombinant expression in Lactococcus lactis subsp. cremoris strains MG1363 or NZ3900 and Lactobacillus plantarum strain WCFS1 using food-grade vectors Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
additional information evaluation of the food grade expression systems NICE, Lactococcus lactis, and pSIP, Lactobacillus plantarum, for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum that also satisfies food safety requirements. Both systems are suitable for 2,5-DKG reductase expression, maximum production yields are obtained with Lactobacillus plantarum/pSIP609 by pH control at 6.5, overview Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,5-didehydro-D-gluconate + NADPH + H+ Corynebacterium glutamicum
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2-dehydro-D-gluconate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum H9CWC0 gene dkr
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-didehydro-D-gluconate + NADPH + H+
-
Corynebacterium glutamicum 2-dehydro-D-gluconate + NADP+
-
?

Synonyms

Synonyms Comment Organism
2,5-diketo-D-gluconic acid reductase
-
Corynebacterium glutamicum
2,5-DKG reductase
-
Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Corynebacterium glutamicum

General Information

General Information Comment Organism
physiological function 2,5-diketo-D-gluconic acid reductase catalyses the reduction of 2,5-diketo-D-gluconic acid to 2-keto-L-gulonic acid, a direct precursor (lactone) of L-ascorbic acid (vitamin C). This reaction is an essential step in the biocatalytic production of the food supplement vitamin C from D-glucose or D-gluconic acid Corynebacterium glutamicum