Protein Variants | Comment | Organism |
---|---|---|
additional information | erg27 mutation and erg27 polymorphism in different strains and fenhexamid phenotypes, overview | Botrytis cinerea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | - |
Botrytis cinerea | |
dimethyl sulfoxide | - |
Botrytis cinerea | |
ethanol | - |
Botrytis cinerea | |
fenhexamid | Erg27 mutations causing amino acid substitutions in or near the transmembrane domain strongly decrease the affinity of fenhexamid for 3-ketoreductase. Sterol 3-ketoreductase sensitivity to fenhexamid in the various fungal strains, overview | Botrytis cinerea | |
propan-2-ol | - |
Botrytis cinerea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Botrytis cinerea | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Botrytis cinerea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Botrytis cinerea | - |
gene erg27 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Botrytis cinerea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4alpha-methylfecosterone + NADPH + H+ | - |
Botrytis cinerea | 4alpha-methylfecosterol + NADP+ | 4alpha-methylfecosterol is further converted to ergosterol | ? | |
fecosterone + NADPH + H+ | - |
Botrytis cinerea | fecosterol + NADP+ | - |
? | |
additional information | substrate for 3-ketoreductase in the first sterol C-4 demethylation cycle is 4alpha-methylfecosterone, whereas that for the second C-4 demethylation cycle is fecosterone | Botrytis cinerea | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sterol 3-ketoreductase | - |
Botrytis cinerea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Botrytis cinerea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Botrytis cinerea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Botrytis cinerea |
General Information | Comment | Organism |
---|---|---|
malfunction | two Botrytis cinerea fenhexamid-resistant phenotypes both result from mutations in the erg27 gene encoding 3-ketoreductase. Erg27 mutations causing amino acid substitutions in or near the transmembrane domain strongly decrease the affinity of fenhexamid for 3-ketoreductase | Botrytis cinerea |
metabolism | the 3-ketoreductase is involved at C-4 demethylation steps during ergosterol biosynthesis. Steryl compound contents of Botrytis strains with different fenhexamid susceptibilities, overview | Botrytis cinerea |