Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | both directions | Agama stellio stellio | |
Cu2+ | both directions | Agama stellio stellio | |
glutamate | both directions | Agama stellio stellio | |
Mg2+ | both directions | Agama stellio stellio | |
Mn2+ | both directions | Agama stellio stellio | |
oxalate | both directions | Agama stellio stellio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NAD+ | pH 7.5, 25°C | Agama stellio stellio | |
0.02 | - |
pyruvate | pH 7.5, 25°C | Agama stellio stellio | |
0.04 | - |
NADH | pH 7.5, 25°C | Agama stellio stellio | |
8.1 | - |
(S)-lactate | pH 7.5, 25°C | Agama stellio stellio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, SDS-PAGE | Agama stellio stellio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agama stellio stellio | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoenzyme LDH-5 | Agama stellio stellio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Agama stellio stellio | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
53 | - |
- |
Agama stellio stellio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | - |
Agama stellio stellio | (S)-lactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE | Agama stellio stellio |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
30 min, stable | Agama stellio stellio |
90 | - |
rapid inactivation | Agama stellio stellio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
with pyruvate as substrate | Agama stellio stellio |
9.2 | - |
with lactate as substrate | Agama stellio stellio |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.4 | 10.8 | pH 5.4: about 60% of maximal activity, pH 10.8: about 55% of maximal activity, reaction with pyruvate | Agama stellio stellio |
6.6 | 10.8 | pH 5.4: about 55% of maximal activity, pH 10.8: about 55% of maximal activity, reaction with lactate | Agama stellio stellio |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Agama stellio stellio | |
NADH | - |
Agama stellio stellio |