Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-diphosphate | L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Lactococcus lactis | |
pyruvate | above 20 mM | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
NADH | pH 6.9, 90 mM Tris-maleate buffer, pH 6.9, 0.5 mM fructose 1,6-diphosphate | Lactococcus lactis | |
1.5 | - |
pyruvate | pH 6.9, 90 mM Tris-maleate buffer, pH 6.9, 0.5 mM fructose 1,6-diphosphate | Lactococcus lactis | |
2.4 | - |
NAD+ | in presence of 1 mM fructose 1,6-diphosphate | Lactococcus lactis | |
8 | - |
NADH | without fructose 1,6-diphosphate | Lactococcus lactis | |
100 | - |
(S)-lactate | in presence of 1 mM fructose 1,6-diphosphate | Lactococcus lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | no effect | Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Lactococcus lactis | the enzyme is unlikely to catalyze lactate oxidation at an appreciable rate under physiological conditions | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
109 | - |
- |
Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | inactive towards D-lactate | Lactococcus lactis | pyruvate + NADH + H+ | - |
r | |
additional information | the enzyme is unlikely to catalyze lactate oxidation at an appreciable rate under physiological conditions | Lactococcus lactis | ? | - |
? | |
pyruvate + NADH + H+ | - |
Lactococcus lactis | (S)-lactate + NAD+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
in presence of fructose 1,6-diphosphate | Lactococcus lactis |
8.2 | - |
in absence of fructose 1,6-diphosphate | Lactococcus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | coenzyme | Lactococcus lactis | |
NADH | coenzyme | Lactococcus lactis |