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Literature summary for 1.1.1.27 extracted from

  • Eichner, R.D.
    L(+)-lactate dehydrogenase from Homarus americanus (1982), Methods Enzymol., 89, 359-362.
No PubMed abstract available
Search for more literature for 1.1.1.27

Inhibitors

Inhibitors Comment Organism Structure
DTNB
-
 Homarus americanus
p-hydroxymercuribenzoate
-
 Homarus americanus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 NADH
-
 Homarus americanus
0.24
-
 pyruvate
-
 Homarus americanus
1.1
-
 NAD+
-
 Homarus americanus
1100
-
 (S)-lactate
-
 Homarus americanus

Organism

Organism UniProt Comment Textmining
Homarus americanus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homarus americanus

Source Tissue

Source Tissue Comment Organism Textmining
muscle tail muscle Homarus americanus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homarus americanus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
 Homarus americanus pyruvate + NADH + H+
-
 r
pyruvate + NADH + H+
-
 Homarus americanus (S)-lactate + NAD+
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2330
-
 NADH
-
 Homarus americanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 reduction of pyruvate and oxidation of lactate Homarus americanus

Cofactor

Cofactor Comment Organism Structure
NAD+ coenzyme Homarus americanus
NADH coenzyme Homarus americanus