Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-diphosphate | L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate | Staphylococcus epidermidis | |
fructose 1,6-diphosphate | concentration required for 50% maximal activity is about 0.15 mM | Staphylococcus epidermidis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive with NADH | Staphylococcus epidermidis | |
AMP | - |
Staphylococcus epidermidis | |
ATP | - |
Staphylococcus epidermidis | |
GTP | - |
Staphylococcus epidermidis | |
oxamate | - |
Staphylococcus epidermidis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, SDS-PAGE | Staphylococcus epidermidis |
130000 | - |
sucrose density gradient centrifugation | Staphylococcus epidermidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus epidermidis | - |
ATCC 14990 | - |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus epidermidis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2220 | - |
- |
Staphylococcus epidermidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | poor reaction | Staphylococcus epidermidis | pyruvate + NADH + H+ | - |
r | |
pyruvate + NADH + H+ | - |
Staphylococcus epidermidis | (S)-lactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 36000, SDS-PAGE | Staphylococcus epidermidis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
- |
Staphylococcus epidermidis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
2 min, 6% loss of activity in presence of fructose 1,6-diphosphate, NADH and pyruvate, complete loss of activity without protectant | Staphylococcus epidermidis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
reduction of pyruvate | Staphylococcus epidermidis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 7.2 | pH 5.2: about 35% of maximal activity, pH 7.2: about 50% of maximal activity | Staphylococcus epidermidis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
highest stability at | Staphylococcus epidermidis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | coenzyme | Staphylococcus epidermidis | |
NADH | coenzyme | Staphylococcus epidermidis |