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Literature summary for 1.1.1.268 extracted from

  • Clark, D.D.; Ensign, S.A.
    Characterization of the 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase from Xanthobacter Strain Py2: Product inhibition, pH dependence of kinetic parameters, site-directed mutagenesis, rapid equilibrium inhibition, and chemical modification (2002), Biochemistry, 41, 2727-2740.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Xanthobacter sp.

Protein Variants

Protein Variants Comment Organism
K159A inactive enzyme Xanthobacter sp.
S142A greatly reduced activity Xanthobacter sp.
S142C inactive enzyme Xanthobacter sp.
Y155E inactive enzyme Xanthobacter sp.
Y155F inactive enzyme Xanthobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
2-(2-methyl-2-hydroxypropylthio)ethanesulfonate
-
 Xanthobacter sp.
2-(S)-hydroxypropyl-CoM
-
 Xanthobacter sp.
2-oxopropyl-CoM mixed-type inhibitor with respect to 2-(R)-hydroxypropyl-CoM Xanthobacter sp.
ethanesulfonate
-
 Xanthobacter sp.
mercaptoethanesulfonate
-
 Xanthobacter sp.
methanesulfonate
-
 Xanthobacter sp.
NADH mixed-type inhibitor with respect to 2-(R)-hydroxypropyl-CoM, competitive inhibition with respect to 2-oxopropyl-CoM Xanthobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63900
-
 gel filtration Xanthobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-(R)-hydroxypropyl-CoM + NAD+ Xanthobacter sp.
-
 2-oxopropyl-CoM + NADH
-
 r
2-(R)-hydroxypropyl-CoM + NAD+ Xanthobacter sp. Py2
-
 2-oxopropyl-CoM + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Xanthobacter sp.
-
-
-
Xanthobacter sp. Py2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, recombinant enzyme Xanthobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(2-hydroxyethylthio)ethanesulfonate + NAD+
-
 Xanthobacter sp. 2-(2-oxoethylthio)ethanesulfonate + NADH
-
 ?
2-(2-hydroxyethylthio)ethanesulfonate + NAD+
-
 Xanthobacter sp. Py2 2-(2-oxoethylthio)ethanesulfonate + NADH
-
 ?
2-(R)-hydroxypropyl-CoM + NAD+
-
 Xanthobacter sp. 2-oxopropyl-CoM + NADH
-
 r
2-(R)-hydroxypropyl-CoM + NAD+
-
 Xanthobacter sp. Py2 2-oxopropyl-CoM + NADH
-
 r

Subunits

Subunits Comment Organism
dimer
-
 Xanthobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 2-(2-hydroxyethylthio)ethanesulfonate
-
 Xanthobacter sp.
24.5
-
 2-oxopropyl-CoM
-
 Xanthobacter sp.
25.8
-
 2-(R)-hydroxypropyl-CoM
-
 Xanthobacter sp.

Cofactor

Cofactor Comment Organism Structure
2-mercaptoethanesulfonate
-
 Xanthobacter sp.
NAD+
-
 Xanthobacter sp.
NADH
-
 Xanthobacter sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.156
-
 2-(S)-hydroxypropyl-CoM competitive inhibition with respect to 2-(R)-hydroxypropyl-CoM Xanthobacter sp.
0.406
-
 2-(2-methyl-2-hydroxypropylthio)ethanesulfonate competitive inhibition with respect to 2-(R)-hydroxypropyl-CoM Xanthobacter sp.
2.25
-
 mercaptoethanesulfonate mixed-type inhibition with respect to 2-(R)-hydroxypropyl-CoM Xanthobacter sp.
26.7
-
 ethanesulfonate mixed-type inhibition with respect to 2-(R)-hydroxypropyl-CoM Xanthobacter sp.
147.8
-
 methanesulfonate mixed-type inhibition with respect to 2-(R)-hydroxypropyl-CoM Xanthobacter sp.