Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Xanthobacter sp. |
Protein Variants | Comment | Organism |
---|---|---|
K159A | inactive enzyme | Xanthobacter sp. |
S142A | greatly reduced activity | Xanthobacter sp. |
S142C | inactive enzyme | Xanthobacter sp. |
Y155E | inactive enzyme | Xanthobacter sp. |
Y155F | inactive enzyme | Xanthobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(2-methyl-2-hydroxypropylthio)ethanesulfonate | - |
Xanthobacter sp. | |
2-(S)-hydroxypropyl-CoM | - |
Xanthobacter sp. | |
2-oxopropyl-CoM | mixed-type inhibitor with respect to 2-(R)-hydroxypropyl-CoM | Xanthobacter sp. | |
ethanesulfonate | - |
Xanthobacter sp. | |
mercaptoethanesulfonate | - |
Xanthobacter sp. | |
methanesulfonate | - |
Xanthobacter sp. | |
NADH | mixed-type inhibitor with respect to 2-(R)-hydroxypropyl-CoM, competitive inhibition with respect to 2-oxopropyl-CoM | Xanthobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63900 | - |
gel filtration | Xanthobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(R)-hydroxypropyl-CoM + NAD+ | Xanthobacter sp. | - |
2-oxopropyl-CoM + NADH | - |
r | |
2-(R)-hydroxypropyl-CoM + NAD+ | Xanthobacter sp. Py2 | - |
2-oxopropyl-CoM + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthobacter sp. | - |
- |
- |
Xanthobacter sp. Py2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant enzyme | Xanthobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(2-hydroxyethylthio)ethanesulfonate + NAD+ | - |
Xanthobacter sp. | 2-(2-oxoethylthio)ethanesulfonate + NADH | - |
? | |
2-(2-hydroxyethylthio)ethanesulfonate + NAD+ | - |
Xanthobacter sp. Py2 | 2-(2-oxoethylthio)ethanesulfonate + NADH | - |
? | |
2-(R)-hydroxypropyl-CoM + NAD+ | - |
Xanthobacter sp. | 2-oxopropyl-CoM + NADH | - |
r | |
2-(R)-hydroxypropyl-CoM + NAD+ | - |
Xanthobacter sp. Py2 | 2-oxopropyl-CoM + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Xanthobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
2-(2-hydroxyethylthio)ethanesulfonate | - |
Xanthobacter sp. | |
24.5 | - |
2-oxopropyl-CoM | - |
Xanthobacter sp. | |
25.8 | - |
2-(R)-hydroxypropyl-CoM | - |
Xanthobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanesulfonate | - |
Xanthobacter sp. | |
NAD+ | - |
Xanthobacter sp. | |
NADH | - |
Xanthobacter sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.156 | - |
2-(S)-hydroxypropyl-CoM | competitive inhibition with respect to 2-(R)-hydroxypropyl-CoM | Xanthobacter sp. | |
0.406 | - |
2-(2-methyl-2-hydroxypropylthio)ethanesulfonate | competitive inhibition with respect to 2-(R)-hydroxypropyl-CoM | Xanthobacter sp. | |
2.25 | - |
mercaptoethanesulfonate | mixed-type inhibition with respect to 2-(R)-hydroxypropyl-CoM | Xanthobacter sp. | |
26.7 | - |
ethanesulfonate | mixed-type inhibition with respect to 2-(R)-hydroxypropyl-CoM | Xanthobacter sp. | |
147.8 | - |
methanesulfonate | mixed-type inhibition with respect to 2-(R)-hydroxypropyl-CoM | Xanthobacter sp. |