Application | Comment | Organism |
---|---|---|
drug development | because the enzyme is absent in humans, DXR is a target for drug discovery | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expression of an N-terminal His6-tagged enzyme variant comprising the first 389 of 413 residues | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an N-terminal His6-tagged enzyme variant comprising the first 389 of 413 residues, the mutant is more stable and kinetically similar to the wild-type enzyme | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | product inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | transient kinetics and kinetic mechanism, stopped-flow fluorescence measurements, overview. Kinetics of pre-steady-state reactions and steady-state kinetics, comparison of simulated and experimental values, burst kinetics model for pre-steady-state kinetics | Mycobacterium tuberculosis | |
0.0098 | - |
NADPH | pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate | Mycobacterium tuberculosis | |
0.084 | - |
2-C-methyl-D-erythritol 4-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
0.115 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
0.42 | - |
NADP+ | pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Mycobacterium tuberculosis | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
no activity in Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | random substrate binding and ordered release of NADP+ followed by partially rate-limiting release of 2-C-methyl-D-erythritol 4-phosphate, transient generation of MtDXR-NADPH-MEP ternary complex, kinetic analysis and modeling, overview | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Mycobacterium tuberculosis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | NADPH and 2-C-methyl-D-erythritol 4-phosphate bind to the free enzyme and the two bind together to generate a nonproductive ternary complex | Mycobacterium tuberculosis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-deoxy-D-xylulose-5-phosphate reductoisomerase | - |
Mycobacterium tuberculosis |
DXP reductoisomerase | - |
Mycobacterium tuberculosis |
DXR | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.95 | - |
2-C-methyl-D-erythritol 4-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
0.95 | - |
NADP+ | pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate | Mycobacterium tuberculosis | |
5.25 | - |
NADPH | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
5.25 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.7 | - |
NADP+ | pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate | Mycobacterium tuberculosis | |
11.3 | - |
2-C-methyl-D-erythritol 4-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
46 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
540 | - |
NADPH | pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate | Mycobacterium tuberculosis |