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Literature summary for 1.1.1.264 extracted from

  • Adachi, O.; Shinagawa, E.; Matsushita, K.; Ameyama, M.
    Crystallization and properties of 5-keto-D-gluconate reductase from Gluconobacter suboxydans (1979), Agric. Biol. Chem., 43, 75-83.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
55% ammonium sulfate precipitation Gluconobacter oxydans

General Stability

General Stability Organism
highly unstable, 75% loss of activity within a few days, stabilizing agents during purification: D-gluconate, 5-keto-D-gluconate Gluconobacter oxydans

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
Gluconobacter oxydans
Cs2+
-
Gluconobacter oxydans
Cu2+
-
Gluconobacter oxydans
Hg2+
-
Gluconobacter oxydans
Ni2+
-
Gluconobacter oxydans
p-chloromercuribenzoate
-
Gluconobacter oxydans
Sn2+
-
Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
NADPH
-
Gluconobacter oxydans
0.02
-
NADP+
-
Gluconobacter oxydans
0.9
-
5-Keto-D-gluconate
-
Gluconobacter oxydans
20
-
D-gluconate
-
Gluconobacter oxydans

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ slight activation at 1 mM Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
4 * 25000, SDS-PAGE Gluconobacter oxydans
110000
-
gel filtration Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-idonate + NADP+ Gluconobacter oxydans
-
5-dehydro-D-gluconate + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, crystalline, chromatography techniques Gluconobacter oxydans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Gluconobacter oxydans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
69.7
-
-
Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-keto-D-fructose + NADP+ low activity Gluconobacter oxydans ? + NADPH
-
?
D-gluconate + NADP+
-
Gluconobacter oxydans ? + NADPH
-
r
L-idonate + NADP+
-
Gluconobacter oxydans 5-dehydro-D-gluconate + NADPH
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 25000, SDS-PAGE Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
5-keto-D-gluconate reduction Gluconobacter oxydans
10
-
D-gluconate oxidation Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 80% of maximal activity within Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Gluconobacter oxydans