Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity of AFR is not influenced by 1 mM EDTA | Ensifer adhaerens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Ensifer adhaerens |
General Stability | Organism |
---|---|
the highest activity of AFR is measured at 30°C in 100 mM Bistris-HCl at pH 6.5, with 50% remaining activity at pH 5.2 (in citrate buffer) and pH 8.8 (in Tris-HCl buffer) | Ensifer adhaerens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | activity of AFR was not influenced by 1 mM EDTA or by any of the given metal salts NaCl, KCl, MgCl2, CaCl2, MnCl2, FeCl3, and ZnCl2, 1 mM each | Ensifer adhaerens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
NADPH | - |
Ensifer adhaerens | |
8.4 | - |
1,5-Anhydro-D-fructose | - |
Ensifer adhaerens | |
11 | - |
D-glucosone | - |
Ensifer adhaerens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | activity of AFR was not influenced by NaCl, KCl, MgCl2, CaCl2, MnCl2, FeCl3, and ZnCl2, 1 mM each | Ensifer adhaerens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35100 | - |
MALDI-TOF-MS | Ensifer adhaerens |
38200 | - |
gel filtration | Ensifer adhaerens |
40000 | - |
SDS-PAGE | Ensifer adhaerens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ensifer adhaerens | - |
- |
- |
Ensifer adhaerens S-30.7.5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose HP column chromatography, Superdex 200 HR10 30 column chromatography and Red Sepharose CL-6B chromatography | Ensifer adhaerens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
AFR of S. morelense S-30.7.5 was inactive toward pyridine-3-aldehyde, 2,3-butanedione, glucuronic acid, acetaldehyde, and formaldehyde | Ensifer adhaerens |
0.01 | - |
1,5-anhydro-D-glucitol as substrate | Ensifer adhaerens |
0.06 | - |
1,5-anhydro-D-mannitol as substrate | Ensifer adhaerens |
6.5 | - |
in crude cell extract | Ensifer adhaerens |
21 | - |
ascopyrone as substrate | Ensifer adhaerens |
21 | - |
D-galactosone as substrate | Ensifer adhaerens |
53 | - |
D-allosone as substrate | Ensifer adhaerens |
88 | - |
D-xylosone as substrate | Ensifer adhaerens |
118 | - |
6-deoxy-D-glucosone as substrate | Ensifer adhaerens |
118 | - |
D-glucosone as substrate | Ensifer adhaerens |
191 | - |
3-keto-1,5-anhydro-D-fructose as substrate | Ensifer adhaerens |
488.9 | - |
after 75 fold purification | Ensifer adhaerens |
530 | - |
1,5-anhydro-D-fructose as substrate | Ensifer adhaerens |
Storage Stability | Organism |
---|---|
-20°C, 50 days, 50% loss of activity | Ensifer adhaerens |
0°C, 50 days, 50% loss of activity | Ensifer adhaerens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-fructose + NADP+ | - |
Ensifer adhaerens | 1,5-anhydro-D-mannitol + NADPH | - |
r | |
1,5-anhydro-D-fructose + NADP+ | - |
Ensifer adhaerens S-30.7.5 | 1,5-anhydro-D-mannitol + NADPH | - |
r | |
1,5-anhydro-D-glucitol + NADPH | - |
Ensifer adhaerens | ? + NADP+ | - |
? | |
1,5-anhydro-D-glucitol + NADPH | - |
Ensifer adhaerens S-30.7.5 | ? + NADP+ | - |
? | |
1,5-anhydro-D-mannitol + NADPH | - |
Ensifer adhaerens | ? + NADP+ | - |
? | |
1,5-anhydro-D-mannitol + NADPH | - |
Ensifer adhaerens S-30.7.5 | ? + NADP+ | - |
? | |
3-keto-1,5-anhydro-D-fructose + NADP+ | - |
Ensifer adhaerens | 1,5-anhydro-D-mannitol + NADPH | - |
? | |
3-keto-1,5-anhydro-D-fructose + NADP+ | - |
Ensifer adhaerens S-30.7.5 | 1,5-anhydro-D-mannitol + NADPH | - |
? | |
6-deoxy-D-glucosone + NADP+ | - |
Ensifer adhaerens | 6-deoxy-D-mannose + NADPH | - |
? | |
6-deoxy-D-glucosone + NADP+ | - |
Ensifer adhaerens S-30.7.5 | 6-deoxy-D-mannose + NADPH | - |
? | |
ascopyrone P + NADP+ | - |
Ensifer adhaerens | ? + NADPH | - |
? | |
D-allosone + NADP+ | - |
Ensifer adhaerens | D-altrose + NADPH | - |
? | |
D-galactosone + NADP+ | - |
Ensifer adhaerens | D-talose + NADPH | - |
? | |
D-glucosone + NADP+ | - |
Ensifer adhaerens | D-mannose + NADPH | - |
? | |
D-xylosone + NADP+ | - |
Ensifer adhaerens | D-lyxose + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35100, MALDI-TOF-MS | Ensifer adhaerens |
Synonyms | Comment | Organism |
---|---|---|
AFR | - |
Ensifer adhaerens |
NADPH-dependent monomeric reductase | - |
Ensifer adhaerens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
63.2 | - |
D-glucosone | - |
Ensifer adhaerens | |
286.7 | - |
1,5-Anhydro-D-fructose | - |
Ensifer adhaerens | |
1300 | - |
1,5-Anhydro-D-fructose | - |
Ensifer adhaerens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.2 | 8.8 | at 30°C the highest activity of AFR is measured in 100 mM Bistris-HCl at pH 6.5, with 50% remaining activity at pH 5.2 (in citrate buffer) and pH 8.8 (in Tris-HCl buffer) | Ensifer adhaerens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Ensifer adhaerens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ensifer adhaerens | - |
- |
4.3 |