Protein Variants | Comment | Organism |
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additional information | generation of the C-terminally Ile274-truncated variant T4HNR(1-273) | Pyricularia grisea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
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1,3,6,8-tetrahydroxynaphthalene + NADPH + H+ | Pyricularia grisea | reduction reaction via the via the 3-keto tautomer of 1,3,6,8-tetrahydroxynaphthalene | scytalone + NADP+ | - |
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Organism | UniProt | Comment | Textmining |
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Pyricularia grisea | - |
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Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3,6,8-tetrahydroxynaphthalene + NADPH + H+ | reduction reaction via the via the 3-keto tautomer of 1,3,6,8-tetrahydroxynaphthalene | Pyricularia grisea | scytalone + NADP+ | - |
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1,3,6,8-tetrahydroxynaphthalene + NADPH + H+ | with enatiomeric excess of over 95%, reduction reaction via the via the 3-oxo tautomer of 1,3,6,8-tetrahydroxynaphthalene | Pyricularia grisea | scytalone + NADP+ | - |
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1,3,6-trihydroxynaphthalene + NADPH + H+ | - |
Pyricularia grisea | (R)-8-deoxyscytalone + NADP+ | - |
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1,3-dihydroxynaphthalene + NADPH + H+ | very low activity | Pyricularia grisea | 3-hydroxy-3,4-dihydronaphthalen-1(2H)-one + NADP+ | - |
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2-methylcyclohexanone + NADPH + H+ | - |
Pyricularia grisea | trans-2-methylcyclohexanol + NADP+ | - |
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2-tetralone + NADPH + H+ | high activity, almost complete conversion | Pyricularia grisea | 2-tetralol + NADP+ | - |
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5-hydroxy-2-tetralone + NADPH + H+ | - |
Pyricularia grisea | 5-hydroxy-2-tetralol + NADP+ | - |
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5-methoxy-2-tetralone + NADPH + H+ | high activity, almost complete conversion | Pyricularia grisea | 5-methoxy-2-tetralol + NADP+ | - |
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6-hydroxy-2-tetralone + NADPH + H+ | - |
Pyricularia grisea | 6-hydroxy-2-tetralol + NADP+ | - |
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6-methoxy-2-tetralone + NADPH + H+ | - |
Pyricularia grisea | 6-methoxy-2-tetralol + NADP+ | - |
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7-hydroxy-2-tetralone + NADPH + H+ | high activity, almost complete conversion | Pyricularia grisea | 7-hydroxy-2-tetralol + NADP+ | - |
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7-methoxy-2-tetralone + NADPH + H+ | high activity, almost complete conversion | Pyricularia grisea | 7-methoxy-2-tetralol + NADP+ | - |
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8-hydroxy-2-tetralone + NADPH + H+ | high activity, almost complete conversion | Pyricularia grisea | 8-hydroxy-2-tetralol + NADP+ | - |
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8-methoxy-2-tetralone + NADPH + H+ | high activity, almost complete conversion | Pyricularia grisea | 8-methoxy-2-tetralol + NADP+ | - |
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Synonyms | Comment | Organism |
---|---|---|
T4HNR | - |
Pyricularia grisea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pyricularia grisea |
General Information | Comment | Organism |
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evolution | the enzyme is a member of the short-chain dehydrogenase/reductase (SDR) family of enzymes | Pyricularia grisea |
metabolism | the enzyme catalyzes a step in 1,8-dihydroxynaphthalene-melanin biosynthesis, overview | Pyricularia grisea |
additional information | structure-activity relationship of T4HNR and dynamics of its catalytic cycle, substrate docking study, the carboxylate of Ile274 might have an impact on substrate orientation, overview. Two orientations of tetralones in the active site of T4HNR revealed by docking studies: 1. si-face orientation (representing the preferential hydrophobic interaction of the methoxy group of substrate 5-methoxy-2-tetralone) and 2. the re-face orientation | Pyricularia grisea |