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Literature summary for 1.1.1.236 extracted from

  • Couladis, M.M.; Brent Friesen, J.; Landgrebe, M.E.; Leete, E.
    Enzymes catalysing the reduction of tropinone to tropine and psi-tropine isolated from the roots of Datura innoxia (1991), Phytochemistry, 30, 801-805.
No PubMed abstract available

General Stability

General Stability Organism
denatured enzyme does not catalyze the reduction of tropinone with NADPH Datura inoxia
very unstable Datura stramonium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tropinone + NADPH + H+ Datura inoxia
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Datura stramonium
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Atropa belladonna
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Hyoscyamus niger
-
pseudotropine + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Atropa belladonna
-
-
-
Datura inoxia
-
-
-
Datura stramonium
-
-
-
Hyoscyamus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Datura inoxia
-
Hyoscyamus niger

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Datura inoxia
-
root
-
Datura stramonium
-
root
-
Atropa belladonna
-
root
-
Hyoscyamus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0145
-
-
Datura inoxia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-methyl-4-piperidone + NADPH
-
Datura stramonium N-methylpiperidin-4-ol + NADP+
-
?
N-methyl-4-piperidone + NADPH more efficient substrate, 230% of activity with tropinone Hyoscyamus niger N-methylpiperidin-4-ol + NADP+
-
?
N-methyltropinone + NADPH
-
Datura inoxia N-methyl-3-beta-tropine + NADP+
-
ir
N-propyl-4-piperidone + NADPH
-
Datura stramonium N-propylpiperidin-4-ol + NADP+
-
?
N-propyl-4-piperidone + NADPH more efficient substrate, 300% of activity with tropinone Hyoscyamus niger N-propylpiperidin-4-ol + NADP+
-
?
tropine + NADP+
-
Datura stramonium tropinone + NADPH
-
r
tropinone + NADPH + H+
-
Datura inoxia pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Datura stramonium pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Datura stramonium pseudotropine + NADP+
-
ir
tropinone + NADPH + H+
-
Atropa belladonna pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Hyoscyamus niger pseudotropine + NADP+
-
?
tropinone + NADPH + H+ enzymes fails to catalyze the oxidation of tropine to tropinone in presence of NADP+ Datura inoxia pseudotropine + NADP+
-
ir
tropinone + NADPH + H+
-
Datura stramonium tropine + NADP+ only tropine formed by the reduction of tropinone, no evidence for the formation of psi-tropine r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
tropine oxidation Datura stramonium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Hyoscyamus niger
6.4
-
-
Datura inoxia
6.8
-
tropinone reduction Datura stramonium
9.5
-
tropine oxidation Datura stramonium

Cofactor

Cofactor Comment Organism Structure
NADH less effective by a factor of 3.7 than NADPH Datura stramonium
NADH preference for NADPH, velocity 5.4% of the velocity found with NADPH Hyoscyamus niger
NADH 70-80% reduction in psi tropine forming reductase activity when NADH is substituted for NADPH Datura inoxia
NADP+ oxidation does not occur when NAD+ is substituted for NADP+ Datura stramonium
NADPH
-
Datura stramonium
NADPH
-
Hyoscyamus niger