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Literature summary for 1.1.1.235 extracted from
- 8-Ketodeoxycoformycin and 8-ketocoformycin as intermediates in the biosynthesis of 2-deoxycoformycin and coformycin (1988), Biochemistry, 27, 5790-5795.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
8-Ketocoformycin | - |
Streptomyces antibioticus |  |
| 0.25 | - |
8-Ketodeoxycoformycin | - |
Streptomyces antibioticus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8-ketocoformycin + NADPH | Streptomyces antibioticus | production of the antibiotic coformycin | coformycin + NADP+ | - |
r | |
| 8-ketodeoxycoformycin + NADPH | Streptomyces antibioticus | production of the antibiotic 2'-deoxycoformycin | 2'-deoxycoformycin + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces antibioticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Streptomyces antibioticus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| coformycin + NADP+ = 8-oxocoformycin + NADPH + H+ | possible mechanism | Streptomyces antibioticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8-ketocoformycin + NADPH | stereospecifically reduces the 8-keto group of the ketonucleoside substrate to a hydroxyl group with the R configuration at C-8 | Streptomyces antibioticus | coformycin + NADP+ | - |
r | |
| 8-ketocoformycin + NADPH | production of the antibiotic coformycin | Streptomyces antibioticus | coformycin + NADP+ | - |
r | |
| 8-ketodeoxycoformycin + NADPH | stereospecifically reduces the 8-keto group of the ketonucleoside substrate to a hydroxyl group with the R configuration at C-8 | Streptomyces antibioticus | 2'-deoxycoformycin + NADP+ | - |
r | |
| 8-ketodeoxycoformycin + NADPH | production of the antibiotic 2'-deoxycoformycin | Streptomyces antibioticus | 2'-deoxycoformycin + NADP+ | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Streptomyces antibioticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
no activity | Streptomyces antibioticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Streptomyces antibioticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | NADPH is 25-fold higher active than NADH | Streptomyces antibioticus | |
| NADPH | B-specific with respect to NADPH, 25-fold higher activity than with NADH | Streptomyces antibioticus | |
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