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Literature summary for 1.1.1.234 extracted from

  • Stich, K.; Forkmann, G.
    Biosynthesis of 3-deoxyanthocyanins with flower extracts from Sinningia cardinalis (1988), Biochemistry, 27, 785-789.
No PubMed abstract available
Search for more literature for 1.1.1.234

General Stability

General Stability Organism
freezing fresh flowers in liquid nitrogen and storing them at -80°C enhances extractable activity compared to fresh flowers Sinningia cardinalis

Inhibitors

Inhibitors Comment Organism Structure
glycerol 10% v/v, complete irreversible inactivation Sinningia cardinalis
additional information no inhibition by KCN, EDTA, diethyl dithiocarbamate, phenanthroline or N-ethylmaleimide, 2 mM each Sinningia cardinalis
naringenin above 0.1 mM Sinningia cardinalis
p-chloromercuribenzoate 0.5 mM Sinningia cardinalis
sucrose 20% w/v, complete irreversible inactivation Sinningia cardinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021 0.023 (2S)-naringenin
-
 Sinningia cardinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S)-flavanone + NADPH Sinningia cardinalis involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol (2S)-flavan-4-ol + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Sinningia cardinalis
-
 syn. Rechsteineria cardinalis, strain " Feuerschein"
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Sinningia cardinalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
-
 Sinningia cardinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-eriodictyol + NADPH
-
 Sinningia cardinalis luteoforol + NADP+
-
 ?
(2S)-flavanone + NADPH involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol Sinningia cardinalis (2S)-flavan-4-ol + NADP+
-
 ?
(2S)-naringenin + NADPH + H+
-
 Sinningia cardinalis apiforol + NADP+
-
 ?
5,7,3',4',5'-pentahydroxyflavanone + NADPH
-
 Sinningia cardinalis 5,7,3',4',5'-pentahydroxyflavan-4-ol + NADP+
-
 ?
additional information as dihydroxyflavonols, such as dihydrokaempferol, dihydroquercetin and dihydromyricetin are substrates for the Sinningia enzyme preparation, too, the enzyme might be identical with dihydroflavonol-4-reductase Sinningia cardinalis ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Sinningia cardinalis

pH Range

pH Minimum pH Maximum Comment Organism
4.7 6.9 about half-maximal activity at pH 4.7 and 6.9 Sinningia cardinalis

Cofactor

Cofactor Comment Organism Structure
additional information no cofactors are FAD, FMN or tetrahydropterin, with or without NADPH Sinningia cardinalis
NADH 77% as active as NADPH Sinningia cardinalis
NADPH required Sinningia cardinalis