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Literature summary for 1.1.1.231 extracted from
- Isolation and properties of an NADP+-dependent PGI2-specific 15-hydroxyprostaglandin dehydrogenase from rabbit kidney (1982), Methods Enzymol., 86, 152-155.
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing: repeatedly, slow loss of activity | Oryctolagus cuniculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0134 | - |
NADP+ | - |
Oryctolagus cuniculus |  |
| 0.278 | - |
prostaglandin I2 | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
gel filtration | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+ | Oryctolagus cuniculus | - |
(5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.9 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| stable for months when frozen | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+ | - |
Oryctolagus cuniculus | (5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH | - |
? | |
| (5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+ | i.e. prostaglandin I2, prostacyclin | Oryctolagus cuniculus | (5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH | i.e. 15-keto prostaglandin I2 | ? | |
| 6-keto-prostaglandin F1alpha + NADP+ | at 1.8% the reaction rate of prostaglandin I2 | Oryctolagus cuniculus | 6,15-diketoprostaglandin F2alpha + NADPH | - |
? | |
| additional information | enzyme catalyzes reduction of 9-keto group in prostaglandin E2 at less than 1% the rate of prostaglandin I2 oxidation | Oryctolagus cuniculus | ? | - |
? | |
| prostaglandin B1 + NADP+ | at 8% the reaction rate of prostaglandin I2 | Oryctolagus cuniculus | 15-ketoprostaglandin B1 + NADPH + H+ | - |
? | |
| prostaglandin E2 + NADP+ | at 4% the reaction rate of prostaglandin I2 | Oryctolagus cuniculus | 15-ketoprostaglandin E2 + NADPH + H+ | - |
? | |
| prostaglandin F2alpha + NADP+ | at 2.2% the reaction rate of prostaglandin I2 | Oryctolagus cuniculus | 15-ketoprostaglandin F2alpha + NADPH + H+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.3 | - |
assay at | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NAD+ | Oryctolagus cuniculus | |
| NADP+ | - |
Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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