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Literature summary for 1.1.1.23 extracted from

  • Nagai, A.; Scheidegger, A.
    Purification and characterization of histidinol dehydrogenase from cabbage (1991), Arch. Biochem. Biophys., 284, 127-132.
    View publication on PubMed
Search for more literature for 1.1.1.23

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 0.5 mM, 35% inhibition Brassica oleracea
Ca2+ 0.5 mM, 64% inhibition Brassica oleracea
Cu2+ 0.5 mM, 76% inhibition Brassica oleracea
Mg2+ 0.5 mM, 46% inhibition Brassica oleracea
Ni2+ 0.5 mM, 54% inhibition Brassica oleracea
Zn2+ 0.5 mM, 66% inhibition Brassica oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 L-histidinol
-
 Brassica oleracea
0.042
-
 NAD+
-
 Brassica oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.5 mM, 26% increase Brassica oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 alpha,alpha, 2 * 52000, SDS-PAGE Brassica oleracea
103000
-
 gel filtration Brassica oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-histidinal + NAD+ + H2O Asparagus officinalis reaction intermediate L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O Triticum aestivum reaction intermediate L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O Cucumis sativus reaction intermediate L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O Lactuca sativa reaction intermediate L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O Brassica oleracea reaction intermediate L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O Solanum melongena reaction intermediate L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O Rosa sp. reaction intermediate L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O Brassica oleracea var. capitata reaction intermediate L-histidine + NADH + H+
-
 ?
L-histidinol + 2 NAD+ + H2O Asparagus officinalis
-
 L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O Triticum aestivum
-
 L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O Cucumis sativus
-
 L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O Lactuca sativa
-
 L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O Brassica oleracea
-
 L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O Solanum melongena
-
 L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O Rosa sp.
-
 L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O Brassica oleracea var. capitata
-
 L-histidine + 2 NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Asparagus officinalis
-
 asparagus shoots
-
Brassica oleracea
-
-
-
Brassica oleracea var. capitata
-
 spring cabbage
-
Cucumis sativus
-
 cucumber shoots
-
Lactuca sativa
-
 lettuce shoots
-
Rosa sp.
-
 rose cell culture
-
Solanum melongena
-
 eggplant shoots
-
Triticum aestivum
-
 wheat germ
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-chromatography, L-histidinol-Sepharose 4B affinity chromatography Brassica oleracea

Source Tissue

Source Tissue Comment Organism Textmining
germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0003
-
 enriched extracts Cucumis sativus
0.0004
-
 enriched extracts Lactuca sativa
0.0004
-
 enriched extracts Solanum melongena
0.0009
-
 enriched extracts Asparagus officinalis
0.0016
-
 enriched extracts Brassica oleracea
0.0021
-
 enriched extracts Triticum aestivum
0.0061
-
 enriched extracts Brassica oleracea var. capitata
0.0062
-
 enriched extracts Rosa sp.
10.16
-
-
 Brassica oleracea

Storage Stability

Storage Stability Organism
4°C, 1 week, 10% Brassica oleracea
4°C, 20% glycerol, 1 week, 0% Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-histidinal + NAD+ + H2O
-
 Asparagus officinalis L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O
-
 Triticum aestivum L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O
-
 Cucumis sativus L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O
-
 Lactuca sativa L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O
-
 Brassica oleracea L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O
-
 Solanum melongena L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O
-
 Rosa sp. L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O
-
 Brassica oleracea var. capitata L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O reaction intermediate Asparagus officinalis L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O reaction intermediate Triticum aestivum L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O reaction intermediate Cucumis sativus L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O reaction intermediate Lactuca sativa L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O reaction intermediate Brassica oleracea L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O reaction intermediate Solanum melongena L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O reaction intermediate Rosa sp. L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O reaction intermediate Brassica oleracea var. capitata L-histidine + NADH + H+
-
 ?
L-histidinol + 2 NAD+ + H2O
-
 Asparagus officinalis L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O
-
 Triticum aestivum L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O
-
 Cucumis sativus L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O
-
 Lactuca sativa L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O
-
 Brassica oleracea L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O
-
 Solanum melongena L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O
-
 Rosa sp. L-histidine + 2 NADH + 2 H+
-
 ?
L-histidinol + 2 NAD+ + H2O
-
 Brassica oleracea var. capitata L-histidine + 2 NADH + 2 H+
-
 ?

Subunits

Subunits Comment Organism
homodimer alpha,alpha, 2 * 52000, SDS-PAGE Brassica oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
 purified from cabbage heads Brassica oleracea

pH Stability

pH Stability pH Stability Maximum Comment Organism
9.2 10 rapid loss of activity above pH 10 Brassica oleracea

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Asparagus officinalis
NAD+
-
 Triticum aestivum
NAD+
-
 Cucumis sativus
NAD+
-
 Lactuca sativa
NAD+
-
 Brassica oleracea
NAD+
-
 Solanum melongena
NAD+
-
 Rosa sp.
NAD+
-
 Brassica oleracea var. capitata