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Literature summary for 1.1.1.23 extracted from

  • Nagai, A.; Kheirolomoom, A.; Ohta, D.
    Site-directed mutagenesis shows that the conserved cysteine residues of histidinol dehydrogensae are not essential for catalysis (1993), J. Biochem., 114, 856-861.
    View publication on PubMed
Search for more literature for 1.1.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Spodoptera frugiperda, SF9 cells Brassica oleracea

Protein Variants

Protein Variants Comment Organism
C112A conserved Cys-112 is not involved in catalysis, Cys-112 corresponds to Cys-116 in Salmonella typhimurium Brassica oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 L-histidinol
-
 Brassica oleracea
0.053
-
 NAD+
-
 Brassica oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast enzyme contains chloroplast transit peptide sequence Brassica oleracea 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-histidinal + NAD+ + H2O Brassica oleracea reaction intermediate L-histidine + NADH + H+
-
 ?
L-histidinol + 2 NAD+ + H2O Brassica oleracea
-
 L-histidine + 2 NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-histidinal + NAD+ + H2O
-
 Brassica oleracea L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O reaction intermediate Brassica oleracea L-histidine + NADH + H+
-
 ?
L-histidinol + 2 NAD+ + H2O
-
 Brassica oleracea L-histidine + 2 NADH + 2 H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
35
-
 L-histidinol recombinant enzyme without transit peptide Brassica oleracea

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Brassica oleracea