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Literature summary for 1.1.1.229 extracted from

  • Furuichi, A.; Akita, H.; Matsukura, H.; Oishi, T.; Horikoshi, K.
    Purification and properties of an asymmetric reduction of diethyl 2-methyl-3-oxosuccinate in Saccharomyces fermentati (1987), Agric. Biol. Chem., 51, 293-299.
No PubMed abstract available
Search for more literature for 1.1.1.229

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 30% of activity at 10 mM Torulaspora delbrueckii
cysteine complete inhibition at 10 mM Torulaspora delbrueckii
FMN slight inhibition at 0.2 mM Torulaspora delbrueckii
high salt concentration 1M NaCl or KCl Torulaspora delbrueckii
KCN complete inhibition at 10 mM Torulaspora delbrueckii
monoiodoacetate complete inhibition at 10 mM Torulaspora delbrueckii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.25
-
 Diethyl 2-methyl-3-oxosuccinate
-
 Torulaspora delbrueckii
111
-
 Diethyl 2-methyl-3-hydroxysuccinate
-
 Torulaspora delbrueckii

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ strong inhibitor Torulaspora delbrueckii
Cu2+ strong inhibitor Torulaspora delbrueckii
Fe3+ strong inhibitor Torulaspora delbrueckii
K+ strongly inhibitory at 1 M Torulaspora delbrueckii
Na+ strongly inhibitory at 1 M Torulaspora delbrueckii
Zn2+ slight inhibition Torulaspora delbrueckii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 gel filtration Torulaspora delbrueckii
63000
-
 SDS-PAGE Torulaspora delbrueckii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diethyl 2-methyl-3-oxosuccinate + NADPH Torulaspora delbrueckii asymmetric reduction in yeast, the reduction is strongly preferred over the weak oxidation activity diethyl 2-methyl-3-hydroxysuccinate + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Torulaspora delbrueckii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Torulaspora delbrueckii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.004
-
 crude extract Torulaspora delbrueckii
0.649
-
 purified enzyme Torulaspora delbrueckii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diethyl 2-methyl-3-oxosuccinate + NADPH the reduction is strongly preferred, the catalyzed reaction is an asymmetric reduction, best substrate Torulaspora delbrueckii diethyl 2-methyl-3-hydroxysuccinate + NADP+ alpha-methyl-beta-hydroxy ester, the enzyme catalyzes an asymmetric reduction, the product is a mixture of the (2R,3R)-syn- and (2S,3R)-anti-beta-hydroxyester r
diethyl 2-methyl-3-oxosuccinate + NADPH asymmetric reduction in yeast, the reduction is strongly preferred over the weak oxidation activity Torulaspora delbrueckii diethyl 2-methyl-3-hydroxysuccinate + NADP+
-
 r
dimethyl 2-methyl-3-oxosuccinate + NADPH poor substrate Torulaspora delbrueckii dimethyl 2-methyl-3-hydroxysuccinate + NADP+
-
 ?
additional information no aldehydes or several synthetic substrates are reduced, such as: acetaldehyde, formaldehyde, isobutanal, n-pentanal, n-hexanal, n-heptanal, 4-hydroxybenzaldehyde, 4-dimethylaminobenzaldehyde, benzaldehyde, furfural, 2-methyl-3-oxobutanoic acid benzyl ester, 2-chloro-3-oxobutanoic acid ethyl ester, 3-phenyl-2-oxobutanoic acid methyl ester, 2-methyl-3-oxobutanoic acid ethyl ester, 3-(2'-furyl)-2-methyl-3-oxopropionic acid methyl ester, none of the following alcohols or other substrates is oxidized: methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, n-pentanol, isopentanol, benzyl alcohol, glycerin, shikimic acid, 2-deoxy-D-glucose, glucose 6-phosphate, in the presence of NAD+ or NADP+ Torulaspora delbrueckii ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Torulaspora delbrueckii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Torulaspora delbrueckii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 more than 70% of maximal activity at both temperatures Torulaspora delbrueckii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 very unstable at temperatures above 40°C for 10 min Torulaspora delbrueckii
60
-
 complete loss of activity Torulaspora delbrueckii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 sharp peak Torulaspora delbrueckii

pH Range

pH Minimum pH Maximum Comment Organism
4 8 less than 10% of activity at both pH Torulaspora delbrueckii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 stable for 30 min Torulaspora delbrueckii