Application | Comment | Organism |
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agriculture | transformation of mannose-6-phosphate reductase gene into Arabidopsis and tobacco using Agrobacterium tumefaciens-mediated transformation. Mannose-6-phosphate reductase can act as a selectable marker gene in either a positive or a negative selection mode depending on the plant species. On medium containing 2 g/l mannose, transgenic seeds germinate, whereas wild type seeds did not. Mannose at 30 g/l blanches leaf explants from all 29 transgenic tobacco events with mannose-6-phosphate reductase. In contrast, 30 g/l mannose does not inhibit shoot regeneration from leaf explants of wild-type or transgenic plants with either an antisense mannose-6-phosphate reductase or a plasmid control. Mannose at 30 g/l inhibits seed germination of transgenic tobacco seeds with mannose-6-phosphate reductase but not that of wild-type or transgenic tobacco with either the antisense mannose-6-phosphate reductase or the plasmid control | Apium graveolens |
Organism | UniProt | Comment | Textmining |
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Apium graveolens | - |
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