Literature summary for 1.1.1.22 extracted from

Rocha, J.; Popescu, A.O.; Borges, P.; Mil-Homens, D.; Moreira, L.M.; Sa-Correia, I.; Fialho, A.M.; Frazao, C.
Structure of Burkholderia cepacia UDP-glucose dehydrogenase (UGD) BceC and role of Tyr10 in final hydrolysis of UGD thioester intermediate (2011), J. Bacteriol., 193, 3978-3987.

Search for more literature for 1.1.1.22

Cloned(Commentary)

Cloned (Comment)	Organism
-	Burkholderia cepacia

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 1.75 A resolution	Burkholderia cepacia

Protein Variants

Protein Variants	Comment	Organism
Y10F	mutation in GXGYXG consensus motif, 9% residual activity. Tyr10 plays a catalytic role in the final hydrolysis step. Upon release of NADH after the second oxidation step, Tyr10 may work as a proton conveyer from the aqueous hydrogen-bonding proton wire system to the hydrolytic site	Burkholderia cepacia
Y10K	mutation in GXGYXG consensus motif, 2% residual activity	Burkholderia cepacia
Y10S	mutation in GXGYXG consensus motif, 3% residual activity	Burkholderia cepacia

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.23	-	UDP-alpha-D-glucose	wild-type, pH 8.7, 30°C, Hill-coefficient 0.89	Burkholderia cepacia
0.53	-	NAD+	wild-type, pH 8.7, 30°C, Hill-coefficient 1.5	Burkholderia cepacia

Organism

Organism	UniProt	Comment	Textmining
Burkholderia cepacia	C9E261	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-D-glucose + 2 NAD+ + H2O	-	Burkholderia cepacia	UDP-alpha-D-glucuronate + 2 NADH + 2 H+	-	?

Synonyms

Synonyms	Comment	Organism
BceC	-	Burkholderia cepacia

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Burkholderia cepacia

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
25	-	significant decrease in activity below	Burkholderia cepacia
40	-	70% of maximum activity	Burkholderia cepacia

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
6.7	-	NAD+	wild-type, pH 8.7, 30°C	Burkholderia cepacia
6.9	-	UDP-alpha-D-glucose	wild-type, pH 8.7, 30°C	Burkholderia cepacia

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.7	-	-	Burkholderia cepacia

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	-	37% of maximum activity	Burkholderia cepacia

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Release 2023.1 (January 2023)