Cloned (Comment) | Organism |
---|---|
gene Ugd(BCAL2946) cloned into the pGEM-T Easy vector, sequenced, and ligated into pET29a vector to transform Escherichia coli Top10 cells. Ugd(BCAL2946) expressed in Escherichia coli HMS 174(DE3) | Burkholderia cenocepacia J2315 |
gene Ugd(BCAM0855) cloned into the pGEM-T Easy vector, sequenced, and ligated into pET29a vector to transform Escherichia coli Top10 cells. Ugd(BCAM0855) expressed in Escherichia coli HMS 174(DE3) | Burkholderia cenocepacia J2315 |
gene Ugd(BCAM2034) cloned into the pGEM-T Easy vector, sequenced, and ligated into pET29a vector to transform Escherichia coli Top10 cells. Ugd(BCAM2034) expressed in Escherichia coli BL21 (DE3) | Burkholderia cenocepacia J2315 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | inactivation of gene Ugd(BCAL2946) results in increased sensitivity to polymyxin B and this sensitivity can be overcome when either genes Ugd(BCAL2946) or Ugd(BCAM0855) but not gene ugd(BCAM2034) is expressed from plasmids. Growth of a conditional Ugd(BCAL2946) mutant, created in the DELTAUgd(BCAM0855) background, is significantly impaired under non-permissive conditions. Growth can be rescued by either Ugd(BCAL2946) or Ugd(BCAM0855) expressed in trans, but not by Ugd(BCAM2034) | Burkholderia cenocepacia J2315 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
UDP-glucose | - |
Burkholderia cenocepacia J2315 | |
0.17 | - |
NAD+ | - |
Burkholderia cenocepacia J2315 | |
0.21 | - |
NAD+ | - |
Burkholderia cenocepacia J2315 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50900 | - |
Ugd-FLAG-BCAM2034 | Burkholderia cenocepacia J2315 |
51700 | - |
Ugd-FLAG-BCAL2946 | Burkholderia cenocepacia J2315 |
52100 | - |
Ugd-FLAG-BCAM0855 | Burkholderia cenocepacia J2315 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cenocepacia J2315 | B4EB36 | - |
- |
Burkholderia cenocepacia J2315 | B4EMQ8 | - |
- |
Burkholderia cenocepacia J2315 | B4EN77 | - |
- |
Purification (Comment) | Organism |
---|---|
by nickel affinity and gel filtration | Burkholderia cenocepacia J2315 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | expression of Ugd(BCAL2946) is 5.4- and 135fold greater than that of Ugd(BCAM0855) and Ugd(BCAM2034), respectively. Combined activity of Ugd(BCAL2946) and Ugd(BCAM0855) is essential for the survival of Burkholderia cenocepacia but only the most highly expressed ugd gene, Ugd(BCAL2946), is required for polymyxin B resistance. UDP-galactose, UDP-acetylglucosamine and GDP-mannose are not substrates | Burkholderia cenocepacia J2315 | ? | - |
? | |
additional information | expression of Ugd(BCAL2946) is 5.4- and 135fold greater than that of Ugd(BCAM0855) and Ugd(BCAM2034), respectively. Combined activity of Ugd(BCAL2946) and Ugd(BCAM0855) is essential for the survival of Burkholderia cenocepacia. UDP-galactose, UDP-acetylglucosamine and GDP-mannose are not substrates | Burkholderia cenocepacia J2315 | ? | - |
? | |
additional information | purified Ugd(BCAM2034) shows no in vitro Ugd activity. Expression of Ugd(BCAL2946) is 5.4- and 135fold greater than that of Ugd(BCAM0855) and Ugd(BCAM2034), respectively. UDP-galactose, UDP-acetylglucosamine and GDP-mannose are not substrates | Burkholderia cenocepacia J2315 | ? | - |
? | |
UDP-glucose + 2 NAD+ + H2O | recombinant forms Ugd(BCAL2946) and Ugd(BCAM0855) have similar in vitro Ugd activity | Burkholderia cenocepacia J2315 | UDP-glucuronate + 2 NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | recombinant form of Ugd(BCAL2946) | Burkholderia cenocepacia J2315 |
homodimer | recombinant form of Ugd(BCAM0855) | Burkholderia cenocepacia J2315 |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose dehydrogenase | - |
Burkholderia cenocepacia J2315 |
Ugd | - |
Burkholderia cenocepacia J2315 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.127 | - |
UDP-glucose | - |
Burkholderia cenocepacia J2315 | |
0.133 | - |
UDP-glucose | - |
Burkholderia cenocepacia J2315 | |
0.154 | - |
NAD+ | - |
Burkholderia cenocepacia J2315 | |
0.156 | - |
NAD+ | - |
Burkholderia cenocepacia J2315 |