Protein Variants | Comment | Organism |
---|---|---|
C276A | site-directed mutagenesis, strong decrease in specific activity | Homo sapiens |
C276S | site-directed mutagenesis, strong decrease in specific activity | Homo sapiens |
D280E | site-directed mutagenesis, 3-fold increase in Km for UDP-glucose and a 2-fold reduced Vmax relative to that of the wild type | Homo sapiens |
D280N | site-directed mutagenesis, putative active site residue, mutation severly impairs enzyme function | Homo sapiens |
K220A | site-directed mutagenesis, putative active site residue, mutation severly impairs enzyme function | Homo sapiens |
K220H | site-directed mutagenesis, putative active site residue, mutation severly impairs enzyme function | Homo sapiens |
K220R | site-directed mutagenesis, putative active site residue, mutation severly impairs enzyme function | Homo sapiens |
K279A | site-directed mutagenesis, strong decrease in specific activity | Homo sapiens |
K339A | site-directed mutagenesis, 165fold decrease in affinity for UDP-glucose. Mutant forms a dimer, in contrast to hexameric wild-type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0092 | - |
UDP-glucose | wild-type, pH 7.4, 22°C | Homo sapiens | |
0.022 | - |
UDP-glucose | mutant K339A, pH 7.4, 22°C | Homo sapiens | |
0.42 | - |
NAD+ | wild-type, pH 7.4, 22°C | Homo sapiens | |
0.53 | - |
NAD+ | mutant K339A, pH 7.4, 22°C | Homo sapiens | |
1.5 | - |
UDP-glucose | mutant K339A, pH 7.4, 22°C | Homo sapiens | |
2.1 | - |
NAD+ | mutant K339A, pH 7.4, 22°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000031 | - |
mutant D280N, pH 7.4, 22°C | Homo sapiens |
0.00022 | - |
mutant K276A, pH 7.4, 22°C | Homo sapiens |
0.0004 | - |
mutant K220H, pH 7.4, 22°C | Homo sapiens |
0.00041 | - |
mutant K220R, pH 7.4, 22°C | Homo sapiens |
0.00247 | - |
mutant K279A, pH 7.4, 22°C | Homo sapiens |
0.0027 | - |
mutant K276S, pH 7.4, 22°C | Homo sapiens |
0.0029 | - |
mutant K220A, pH 7.4, 22°C | Homo sapiens |
0.41 | - |
mutant D280E, pH 7.4, 22°C | Homo sapiens |
0.69 | - |
wild-type, 22°C, pH 7.4 | Homo sapiens |
0.74 | - |
mutant K339A, pH 7.4, 22°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2 NAD+ + H2O | - |
Homo sapiens | UDP-glucuronate + 2 NADH + 2 H+ | - |
? |