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Literature summary for 1.1.1.22 extracted from
- Biochemical characterization and phylogenetic analysis of UDP-glucose dehydrogenase from the gellan gum producer Sphingomonas elodea ATCC 31461 (2007), Appl. Microbiol. Biotechnol., 76, 1319-1327.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Sphingomonas elodea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
NAD+ | pH 8.7, 30°C | Sphingomonas elodea |  |
| 0.87 | - |
UDP-glucose | pH 8.7, 30°C | Sphingomonas elodea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48500 | - |
x * 48500, SDS-PAGE and calculated for His-tagged protein | Sphingomonas elodea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingomonas elodea | - |
strain ATCC 31461 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + 2 NAD+ + H2O | - |
Sphingomonas elodea | UDP-glucuronate + 2 NADH + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48500, SDS-PAGE and calculated for His-tagged protein | Sphingomonas elodea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDPG-DH | - |
Sphingomonas elodea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Sphingomonas elodea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
90% of maximum activity | Sphingomonas elodea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.6 | - |
NAD+ | pH 8.7, 30°C | Sphingomonas elodea | |
| 8.73 | - |
UDP-glucose | pH 8.7, 30°C | Sphingomonas elodea | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
- |
Sphingomonas elodea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
46% of maximum acitivity | Sphingomonas elodea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Sphingomonas elodea | |
| NADH | - |
Sphingomonas elodea | |
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Release 2023.1 (January 2023)
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