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Literature summary for 1.1.1.22 extracted from

  • Huh, J.W.; Lee, H.J.; Choi, M.M.; Yang, S.J.; Yoon, S.Y.; Kim, D.W.; Kim, S.Y.; Choi, S.Y.; Cho, S.W.
    Identification of a UDP-glucose-binding site of human UDP-glucose dehydrogenase by photoaffinity labeling and cassette mutagenesis (2005), Bioconjug. Chem., 16, 710-716.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzyme wild-type enzymes in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C276E activity is not measurable at pH 8.7, 22°C Homo sapiens
C276G activity is not measurable at pH 8.7, 22°C Homo sapiens
C276K activity is not measurable at pH 8.7, 22°C Homo sapiens
C276L activity is not measurable at pH 8.7, 22°C Homo sapiens
C276Y activity is not measurable at pH 8.7, 22°C Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information in the presence of 0.12 mM 5-azido-UDP-glucose, 89% of the enzyme activity is lost after 5 min of photolabeling. When the enzyme is photolyzed in presence of 1 mM UDP-glucose, 11% of the enzyme activity is lost. 5-azido-UDP-glucose is photoinserting into a UDP-glucose-binding site on the human enzyme in a specific manner. Uracil, uridine, and glucose have a poor protective effect on the labeling, while UDP and UDP-glucose strongly inhibit photoinsertion Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01703
-
UDP-glucose 22°C, pH 8.7 Homo sapiens
0.05617
-
5-azido-UDP-glucose 22°C, pH 8.7 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-azido-UDP-glucose + NAD+
-
Homo sapiens 5-azido-UDP-glucuronate + NADH + H+
-
?
UDP-glucose + 2 NAD+ + H2O C276 is an active catalytic residue and critically involved in the substrate binding Homo sapiens UDP-glucuronate + 2 NADH + 2 H+
-
?

Synonyms

Synonyms Comment Organism
UGDH
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens