Cloned (Comment) | Organism |
---|---|
expression of mutant enzyme wild-type enzymes in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C276E | activity is not measurable at pH 8.7, 22°C | Homo sapiens |
C276G | activity is not measurable at pH 8.7, 22°C | Homo sapiens |
C276K | activity is not measurable at pH 8.7, 22°C | Homo sapiens |
C276L | activity is not measurable at pH 8.7, 22°C | Homo sapiens |
C276Y | activity is not measurable at pH 8.7, 22°C | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | in the presence of 0.12 mM 5-azido-UDP-glucose, 89% of the enzyme activity is lost after 5 min of photolabeling. When the enzyme is photolyzed in presence of 1 mM UDP-glucose, 11% of the enzyme activity is lost. 5-azido-UDP-glucose is photoinserting into a UDP-glucose-binding site on the human enzyme in a specific manner. Uracil, uridine, and glucose have a poor protective effect on the labeling, while UDP and UDP-glucose strongly inhibit photoinsertion | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01703 | - |
UDP-glucose | 22°C, pH 8.7 | Homo sapiens | |
0.05617 | - |
5-azido-UDP-glucose | 22°C, pH 8.7 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-azido-UDP-glucose + NAD+ | - |
Homo sapiens | 5-azido-UDP-glucuronate + NADH + H+ | - |
? | |
UDP-glucose + 2 NAD+ + H2O | C276 is an active catalytic residue and critically involved in the substrate binding | Homo sapiens | UDP-glucuronate + 2 NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UGDH | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |