Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C276S | no enzymic activity, affinity for NAD+ similar to wild-type, retains predominantly hexameric structure | Homo sapiens |
K279A | no enzymic activity, affinity for NAD+ similar to wild-type, almost exclusively found as dimer | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
UDP-glucose | pH 7.4, 22°C | Homo sapiens | |
0.355 | - |
NAD+ | pH 7.4, 22°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
345000 | - |
gel filtration, dynamic light scattering | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60701 | recombinant His-tagged enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2 NAD+ + H2O | - |
Homo sapiens | UDP-glucuronate + 2 NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 x 57000, SDS-PAGE | Homo sapiens |
More | significant amount of dimeric and monomeric species can be detected | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |