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Literature summary for 1.1.1.22 extracted from

  • Sieberth, V.; Rigg, G.P.; Roberts, I.S.; Jann, K.
    Expression and characterization of UDPGlc dehydrogenase (Kfid), which is encoded in the type-specific region 2 of the Escherichia coli K5 capsule genes (1995), J. Bacteriol., 177, 4562-4565.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
overexpression as glutathione-S-transferase fusion protein in Escherichia coli Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.6
-
 UDPglucose
-
 Escherichia coli
8.4
-
 UDPglucose recombinant glutathione-S-transferase fusion protein Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol recombinant fusion protein Escherichia coli 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 2 * 44000, SDS-PAGE Escherichia coli
72000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + NAD+ + H2O Escherichia coli
-
 UDPglucuronate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant glutathione-S-transferase fusion protein Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + 2 NAD+ + H2O
-
 Escherichia coli UDP-glucuronate + 2 NADH + 2 H+
-
 ?
UDPglucose + NAD+ + H2O
-
 Escherichia coli UDPglucuronate + NADH
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 44000, SDS-PAGE Escherichia coli