Cloned (Comment) | Organism |
---|---|
isozyme MtDFR1, from a young seed cDNA library, functional expression in Escherichia coli | Medicago truncatula |
isozyme MtDFR2, from a young seed cDNA library, functional expression in Escherichia coli | Medicago truncatula |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of the isozyme in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV35S promotor does not lead to increased anthocyanin production in the flowers | Medicago truncatula |
additional information | overexpression of the isozyme in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV35S promotor leads to increased anthocyanin production in the flowers | Medicago truncatula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Medicago truncatula | key enzyme in flux control in biosynthetic branched pathways leading to anthocyanins and condensed tannins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago truncatula | Q6TQT0 | isozyme MtDFR2; cv. Jemalong, 2 isozymes MtDFR1 and MtDFR2 | - |
Medicago truncatula | Q6TQT1 | isozyme MtDFR1; cv. Jemalong, 2 isozymes MtDFR1 and MtDFR2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Medicago truncatula | - |
leaf | - |
Medicago truncatula | - |
additional information | developmental gene expression pattern | Medicago truncatula | - |
seed | - |
Medicago truncatula | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-2,3-dihydrokaempferol + NADPH | - |
Medicago truncatula | cis-3,4-leucopelargonidin + NADP+ | - |
? | |
(+)-2,3-dihydrokaempferol + NADPH | low activity | Medicago truncatula | cis-3,4-leucopelargonidin + NADP+ | - |
? | |
(+/-)-fustin + NADPH | preference for (-)-isomer | Medicago truncatula | ? | - |
? | |
(-)-fustin + NADPH | stereospecific for (-)-isomer | Medicago truncatula | ? | - |
? | |
2,3-dihydromyricetin + NADPH | low activity | Medicago truncatula | cis-3,4-leucodelphinidin + NADP+ | - |
? | |
2,3-dihydroquercetin + NADPH | i.e. (+)-taxifolin, stereospecific for (+)-isomer | Medicago truncatula | cis-3,4-leucocyanidin + NADP+ | - |
? | |
2,3-dihydrorobinetin + NADPH | - |
Medicago truncatula | ? | - |
? | |
additional information | key enzyme in flux control in biosynthetic branched pathways leading to anthocyanins and condensed tannins | Medicago truncatula | ? | - |
? | |
additional information | stereospecific enzyme, no activity with substances lacking the the hydroxyl group at the 3-position or with a double bond present between C2 and C3, e.g. quercetin, apigenin, eriodictyol, and kaempferol | Medicago truncatula | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DFR | - |
Medicago truncatula |
dihydroflavonol 4-reductase | - |
Medicago truncatula |
dihydroflavonol-4-reductase | - |
Medicago truncatula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Medicago truncatula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Medicago truncatula |
7 | - |
- |
Medicago truncatula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Medicago truncatula |