Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant large subunits | Gluconobacter frateurii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Gluconobacter frateurii | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme group of cytochrome C bound to the middle subunit | Gluconobacter frateurii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE | Gluconobacter frateurii |
44000 | - |
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE | Gluconobacter frateurii |
65000 | - |
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE | Gluconobacter frateurii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate + NADP+ | Gluconobacter frateurii | - |
2-dehydro-D-gluconate + NADPH | - |
? | |
D-gluconate + NADP+ | Gluconobacter frateurii IFO 3271 | - |
2-dehydro-D-gluconate + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter frateurii | A4PIB0 | the large subunit is encoded by gene gndL, the small subunit by gene gndS, and the cytochrome C subunit by gene gndC; gene gndL encoding the large subunit of the enzyme | - |
Gluconobacter frateurii IFO 3271 | A4PIB0 | the large subunit is encoded by gene gndL, the small subunit by gene gndS, and the cytochrome C subunit by gene gndC; gene gndL encoding the large subunit of the enzyme | - |
Purification (Comment) | Organism |
---|---|
native enzyme 67fold from membranes and bound to FAD by solubilization with 0.1% Triton X-100, anion chromatography, and three steps of hydrophobic interaction chromatography | Gluconobacter frateurii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
137 | - |
purified native enzyme | Gluconobacter frateurii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate + NADP+ | - |
Gluconobacter frateurii | 2-dehydro-D-gluconate + NADPH | - |
? | |
D-gluconate + NADP+ | the large subunit is the catalytically active enzyme part, high substrate and regiospecificity | Gluconobacter frateurii | 2-dehydro-D-gluconate + NADPH | - |
? | |
D-gluconate + NADP+ | - |
Gluconobacter frateurii IFO 3271 | 2-dehydro-D-gluconate + NADPH | - |
? | |
D-gluconate + NADP+ | the large subunit is the catalytically active enzyme part, high substrate and regiospecificity | Gluconobacter frateurii IFO 3271 | 2-dehydro-D-gluconate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE | Gluconobacter frateurii |
Synonyms | Comment | Organism |
---|---|---|
2-keto-D-gluconate-yielding D-gluconate dehydrogenase | - |
Gluconobacter frateurii |
FAD-GADH | - |
Gluconobacter frateurii |
GADH | - |
Gluconobacter frateurii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gluconobacter frateurii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Gluconobacter frateurii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | bound by the 44 kDa subunit | Gluconobacter frateurii | |
FAD | bound to the enzyme's large 65 kDA subunit | Gluconobacter frateurii | |
NADP+ | - |
Gluconobacter frateurii |