KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state, transient state kinetics, and kinetic isotope effects, kinetic analysis and mechanism, ordered bi-bi mechanism, detailed overview | Rattus norvegicus | |
0.00065 | - |
5alpha-androstane-3,17-dione | pH 7.0, 25, reduction reaction | Rattus norvegicus | |
0.0041 | - |
androsterone | pH 7.0, 25, oxidation reaction | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
androsterone + NADP+ | Rattus norvegicus | - |
5alpha-androstane-3,17-dione + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | bi bi ordered mechanism, kinetic analysis, overview | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
androsterone + NADP+ | - |
Rattus norvegicus | 5alpha-androstane-3,17-dione + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3alpha-HSD | - |
Rattus norvegicus |
AKR1C9 | - |
Rattus norvegicus |
More | cf. EC 1.1.1.50, the enzyme is a member of the aldo-keto reductase superfamily | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
5alpha-androstane-3,17-dione | pH 7.0, 25, reduction reaction | Rattus norvegicus | |
0.82 | - |
androsterone | pH 7.0, 25, oxidation reaction | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | loose association of the NADP(H) is followed by two conformational changes, which increases cofactor affinity by 86fold | Rattus norvegicus | |
NADPH | loose association of the NADP(H) is followed by two conformational changes, which increases cofactor affinity by 86fold | Rattus norvegicus |