Cloned (Comment) | Organism |
---|---|
plasmid DNA containing cDNA is transformed in Escherichia coli HB101, cDNA is used for sequencing | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
1 * 34000 SDS-PAGE | Rattus norvegicus |
37030 | - |
predicted molecular weight from the cDNA | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-hydroxy-5alpha-androstan-3-one + NAD(P)H + H+ | Rattus norvegicus | termination of androgen action | 3alpha,17beta-dihydroxy-5alpha-androstan + NAD(P)+ | - |
? | |
17beta-hydroxy-5alpha-androstan-3-one + NAD(P)H + H+ | Rattus norvegicus Sprangue-Dawley | termination of androgen action | 3alpha,17beta-dihydroxy-5alpha-androstan + NAD(P)+ | - |
? | |
additional information | Rattus norvegicus | involved in biosynthesis of bile acids | ? | - |
? | |
additional information | Rattus norvegicus Sprangue-Dawley | involved in biosynthesis of bile acids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprangue-Dawley | - |
Rattus norvegicus Sprangue-Dawley | - |
Sprangue-Dawley | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | bi bi mechanism, pyridine nucleotide binds before substrate | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
additional information | indomethacin-sensitive 3alpha-hydroxysteroid dehydrogenase widely distributed in prostate, spleen, heart, testis, small intestine, stomach, lung and brain | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-hydroxy-5alpha-androstan-3-one + NAD(P)H + H+ | termination of androgen action | Rattus norvegicus | 3alpha,17beta-dihydroxy-5alpha-androstan + NAD(P)+ | - |
? | |
17beta-hydroxy-5alpha-androstan-3-one + NAD(P)H + H+ | termination of androgen action | Rattus norvegicus Sprangue-Dawley | 3alpha,17beta-dihydroxy-5alpha-androstan + NAD(P)+ | - |
? | |
additional information | metabolizes androgens and glucocorticoids, function as a dihydrodiol dehydrogenase as well as 9-, 11-, and 15-hydroxyprostaglandin dehydrogenase, enzyme catalyzes both oxidation and reduction reaction at physiological pH and is able to reduce aromatic ketones | Rattus norvegicus | ? | - |
? | |
additional information | involved in biosynthesis of bile acids | Rattus norvegicus | ? | - |
? | |
additional information | metabolizes androgens and glucocorticoids, function as a dihydrodiol dehydrogenase as well as 9-, 11-, and 15-hydroxyprostaglandin dehydrogenase, enzyme catalyzes both oxidation and reduction reaction at physiological pH and is able to reduce aromatic ketones | Rattus norvegicus Sprangue-Dawley | ? | - |
? | |
additional information | involved in biosynthesis of bile acids | Rattus norvegicus Sprangue-Dawley | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000 SDS-PAGE | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | dual pyridine nucleotide specificity, cofactor binding-site is located at the NH2 terminus | Rattus norvegicus |