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Literature summary for 1.1.1.210 extracted from

  • Sharaf, M.A.; Sweet, F.
    Dual activity at an enzyme active site: 3beta,20alpha-hydroxysteroid oxidoreductase from fetal blood (1982), Biochemistry, 21, 4615-4620.
    View publication on PubMed

General Stability

General Stability Organism
lyophilization, complete loss of activity Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
17beta-hydroxy-5alpha-androstan-3-one inhibition of 20alpha-reductase activity Bos taurus
19-Nortestosterone 17-bromoacetate competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation Bos taurus
progesterone inhibition of 3beta-reductase activity Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
NADPH 20alpha-reductase Bos taurus
0.0094
-
NADPH 3beta-reductase Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
SDS-PAGE, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
17beta-hydroxy-5alpha-androstan-3-one + NADPH Bos taurus
-
5alpha-androstan-3beta,17beta-diol + NADP+
-
?
progesterone + NADPH Bos taurus
-
20alpha-hydroxy-4-pregnen-3-one + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Reaction

Reaction Comment Organism Reaction ID
5alpha-androstan-3beta,17beta-diol + NADP+ = 17beta-hydroxy-5alpha-androstan-3-one + NADPH + H+ 3beta and 20alpha substrates share the same active site Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte fetal Bos taurus
-

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, indefinitely stable Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17beta-hydoxy-5alpha-androstan-3-one + NADPH
-
Bos taurus 5alpha-androstan-3beta,17beta-diol + NADP+
-
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
Bos taurus 5alpha-androstan-3beta,17beta-diol + NADP+
-
?
progesterone + NADPH
-
Bos taurus 20alpha-hydroxy-4-pregnen-3-one + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Bos taurus
NADPH
-
Bos taurus